公开(公告)号：US20130275349A1

公开(公告)日：2013-10-17

申请号：US13976967

申请日：2011-12-28

申请人： Kei Tashiro ,  Shigeru Kinoshita ,  Tomohito Yagi ,  Masakazu Nakano ,  Kazuhiko Mori ,  Yoko Ikeda ,  Morio Ueno ,  Yuichi Tokuda ,  Katsumi Yagi ,  Kengo Yoshii ,  Masahiro Fuwa

发明人： Kei Tashiro ,  Shigeru Kinoshita ,  Tomohito Yagi ,  Masakazu Nakano ,  Kazuhiko Mori ,  Yoko Ikeda ,  Morio Ueno ,  Yuichi Tokuda ,  Katsumi Yagi ,  Kengo Yoshii ,  Masahiro Fuwa

IPC分类号： G06N99/00

CPC分类号： G06N20/00 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B40/00

摘要： Provided is a technique for determining a physiological attribute in a mammal, including the onset or progression of human glaucoma, with high accuracy. The results of the determination of genotype date and the results of the determination of cytokine date are consolidated by a consolidated determination unit (114); comparison is made for determining as to which is larger, the number of Case determination procedures or the number of control determination procedures (S330); and it is determined as Case (glaucoma) when the number of Case determination procedures is larger and it is determined as Control (normal person) when the number of Control determination procedures is larger.

摘要翻译： 提供了一种用于以高精度确定哺乳动物的生理属性，包括人类青光眼的发病或进展的技术。 确定基因型日期的结果和细胞因子日期的确定结果由综合确定单位（114）合并; 比较确定哪个更大，判定程序的数量或控制确定程序的数量（S330）; 当判定判定过程的数量较多并且当控制确定过程的数量较大时被确定为控制（普通人）时被确定为病例（青光眼）。

公开(公告)号：US09248125B2

公开(公告)日：2016-02-02

申请号：US12675475

申请日：2008-08-28

申请人： Noriko Koizumi ,  Shigeru Kinoshita ,  Morio Ueno

发明人： Noriko Koizumi ,  Shigeru Kinoshita ,  Morio Ueno

IPC分类号： A61K31/4409 ,  A61K31/496 ,  A61K9/00 ,  C07D213/81 ,  C07D401/12 ,  C12N5/079

CPC分类号： A61K31/551 ,  A61K9/0048 ,  A61K31/4409 ,  A61K31/496 ,  C07D213/81 ,  C07D401/12 ,  C12N5/0621 ,  C12N2501/70

摘要： The invention provides an agent for promoting adhesion of a corneal endothelial cell, containing a Rho kinase inhibitor, as well as a culture medium for a corneal endothelial cell, a solution for preservation of cornea, and a method of producing a corneal endothelial preparation, which includes culturing the corneal endothelial cell using the aforementioned culture medium.

摘要翻译： 本发明提供了一种用于促进包含Rho激酶抑制剂的角膜内皮细胞以及角膜内皮细胞培养基，角膜保存用溶液和角膜内皮制剂的制造方法的粘合剂。 包括使用上述培养基培养角膜内皮细胞。

公开(公告)号：US20070178587A1

公开(公告)日：2007-08-02

申请号：US11569414

申请日：2005-05-27

申请人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

发明人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

IPC分类号： C12N5/08 ,  C12N5/06

CPC分类号： G01N33/5073 ,  C12N5/0606 ,  C12N5/0619 ,  C12N2506/02 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058

摘要： The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.

摘要翻译： 本发明提供诱导胚胎干细胞分化的临床适用方法。 具体地，本发明提供培养胚胎干细胞的方法，其包括在羊膜来源的因子存在下培养胚胎干细胞，通过培养方法获得的细胞培养物和胚胎干细胞的培养试剂/培养试剂盒 包括羊膜衍生因子。 本发明还提供一种筛选具有活性作为指标培养胚胎干细胞的活性的羊膜衍生因子和通过筛选方法得到的羊膜细胞衍生因子的方法。 此外，本发明提供了制备用于培养胚胎干细胞的羊膜细胞的方法，通过制备方法获得的羊膜细胞等。

公开(公告)号：US07923246B2

公开(公告)日：2011-04-12

申请号：US11569414

申请日：2005-05-27

申请人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

发明人： Yoshiki Sasai ,  Morio Ueno ,  Shigeru Kinoshita

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071

CPC分类号： G01N33/5073 ,  C12N5/0606 ,  C12N5/0619 ,  C12N2506/02 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5058

摘要： The present invention provides a clinically applicable method of inducing differentiation of the embryonic stem cells. Specifically, the present invention provides a method of culturing embryonic stem cells, which comprises culturing embryonic stem cells in the presence of an amnion-derived factor, a cell culture obtained by the culture method, and a culture agent/culture kit of embryonic stem cells comprising an amnion-derived factor. The present invention also provides a method of screening an amnion-derived factor having an activity useful for culturing embryonic stem cells with the activity as an index, and an amniocyte-derived factor obtained by the screening method. Furthermore, the present invention provides a method of preparing amniocytes useful for culturing embryonic stem cells, amniocytes obtained by the preparation method, and the like.

摘要翻译： 本发明提供诱导胚胎干细胞分化的临床适用方法。 具体地，本发明提供培养胚胎干细胞的方法，其包括在羊膜来源的因子存在下培养胚胎干细胞，通过培养方法获得的细胞培养物和胚胎干细胞的培养试剂/培养试剂盒 包括羊膜衍生因子。 本发明还提供一种筛选具有活性作为指标培养胚胎干细胞的活性的羊膜衍生因子和通过筛选方法得到的羊膜细胞衍生因子的方法。 此外，本发明提供了制备用于培养胚胎干细胞的羊膜细胞的方法，通过制备方法获得的羊膜细胞等。

公开(公告)号：US20100209402A1

公开(公告)日：2010-08-19

申请号：US12675475

申请日：2008-08-28

申请人： Noriko Koizumi ,  Shigeru Kinoshita ,  Morio Ueno

发明人： Noriko Koizumi ,  Shigeru Kinoshita ,  Morio Ueno

IPC分类号： C12N5/071 ,  A01N1/02 ,  A61K35/44

CPC分类号： A61K31/551 ,  A61K9/0048 ,  A61K31/4409 ,  A61K31/496 ,  C07D213/81 ,  C07D401/12 ,  C12N5/0621 ,  C12N2501/70

摘要： The invention provides an agent for promoting adhesion of a corneal endothelial cell, containing a Rho kinase inhibitor, as well as a culture medium for a corneal endothelial cell, a solution for preservation of cornea, and a method of producing a corneal endothelial preparation, which includes culturing the corneal endothelial cell using the aforementioned culture medium.

摘要翻译： 本发明提供了一种用于促进包含Rho激酶抑制剂的角膜内皮细胞以及角膜内皮细胞培养基，角膜保存用溶液和角膜内皮制剂的制造方法的粘合剂。 包括使用上述培养基培养角膜内皮细胞。

公开(公告)号：US11633404B2

公开(公告)日：2023-04-25

申请号：US14879658

申请日：2015-10-09

申请人： Senju Pharmaceutical Co., Ltd. ,  Shigeru Kinoshita

发明人： Noriko Koizumi ,  Shigeru Kinoshita ,  Morio Ueno

IPC分类号： A61K31/551 ,  A61K9/00 ,  A61K31/496 ,  C07D213/81 ,  C07D401/12 ,  C12N5/079 ,  A61K31/4409

摘要： The invention provides an agent for promoting adhesion of a corneal endothelial cell, containing a Rho kinase inhibitor, as well as a culture medium for a corneal endothelial cell, a solution for preservation of cornea, and a method of producing a corneal endothelial preparation, which includes culturing the corneal endothelial cell using the aforementioned culture medium.