公开(公告)号：US11987797B2

公开(公告)日：2024-05-21

申请号：US18057588

申请日：2022-11-21

申请人： LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES

发明人： Haixue Zheng ,  Keshan Zhang ,  Ting Zhang ,  Tao Feng ,  Bo Yang ,  Xing Yang ,  Xijuan Shi ,  Hong Tian ,  Yi Ru ,  Fan Yang ,  Zixiang Zhu ,  Jianhong Guo ,  Jijun He ,  Xiangtao Liu

IPC分类号： C12N15/65 ,  C12N7/00 ,  C12N15/63

CPC分类号： C12N15/65 ,  C12N7/00 ,  C12N15/63 ,  C12N2710/12021 ,  C12N2710/12022

摘要： The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-β and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.