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公开(公告)号：US20240148850A1
公开(公告)日：2024-05-09
申请号：US17997749
申请日：2022-03-17
发明人： Jing ZHANG , Zengjun LU , Kun LI , Pu SUN , Jian WANG , Yimei CAO , Huifang BAO , Zhixun ZHAO , Pinghua LI , Yuanfang FU , Xueqing MA , Hong YUAN , Xingwen BAI , Qiang ZHANG , Dong LI , Zaixin LIU
IPC分类号： A61K39/12 , A61K39/00 , C07K14/005 , G01N33/569
CPC分类号： A61K39/12 , C07K14/005 , G01N33/56983 , A61K2039/5252 , A61K2039/5254 , C12N2770/10022 , C12N2770/10034 , G01N2333/08
摘要： The present disclosure provides an N protein epitope mutation marker for preparing an epitope deletion-marked vaccine strain of type II porcine reproductive and respiratory syndrome virus (PRRSV) and use thereof, belonging to the technical field of biological products. In the mutation marker, one or more amino acids are mutated based on an epitope sequence at positions 92 to 103 of a C-terminal of an N protein of the type II PRRSV; and the epitope mutation marker has an amino acid sequence shown in SEQ ID NO: 1, where X1 is selected from the group consisting of T, P, and A; and X2 is selected from the group consisting of V and A.
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公开(公告)号：US11883411B2
公开(公告)日：2024-01-30
申请号：US17895390
申请日：2022-08-25
发明人： Keshan Zhang , Haixue Zheng , Huimei Cui , Bo Yang , Hong Tian , Zixiang Zhu , Tao Feng , Fan Yang , Weijun Cao , Xusheng Ma , Yi Ru , Jianhong Guo , Xiangtao Liu
IPC分类号： A61K31/5377 , A61P31/20
CPC分类号： A61K31/5377 , A61P31/20
摘要： The present disclosure belongs to the technical field of African swine fever (ASF) treatment, in particular to use of a compound aprepitant in prevention or treatment of the ASF. In the present disclosure, it is found that the compound aprepitant can significantly inhibit replication of an African swine fever virus (ASFV); in addition, it is found that the aprepitant inhibits transcription and protein expression of D1133L, and reduces transcription and protein expression levels of p30 and p72, preventing virus invasion on host cells. Therefore, the compound aprepitant can be used to inhibit early and late infection of the ASFV. Accordingly, the compound aprepitant can be used to prevent or treat the ASF.
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3.发明公开公开(公告)号：US20230270747A1
公开(公告)日：2023-08-31
申请号：US17895390
申请日：2022-08-25
发明人： Keshan ZHANG , Haixue ZHENG , Huimei CUI , Bo YANG , Hong TIAN , Zixiang ZHU , Tao FENG , Fan YANG , Weijun CAO , Xusheng MA , Yi RU , Jianhong GUO , Xiangtao LIU
IPC分类号： A61K31/5377 , A61P31/20
CPC分类号： A61K31/5377 , A61P31/20
摘要： The present disclosure belongs to the technical field of African swine fever (ASF) treatment, in particular to use of a compound aprepitant in prevention or treatment of the ASF. In the present disclosure, it is found that the compound aprepitant can significantly inhibit replication of an African swine fever virus (ASFV); in addition, it is found that the aprepitant inhibits transcription and protein expression of D1133L, and reduces transcription and protein expression levels of p30 and p72, preventing virus invasion on host cells. Therefore, the compound aprepitant can be used to inhibit early and late infection of the ASFV. Accordingly, the compound aprepitant can be used to prevent or treat the ASF.
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4.发明公开公开(公告)号：US20230218736A1
公开(公告)日：2023-07-13
申请号：US17948752
申请日：2022-09-20
发明人： Junjun SHAO , Guanglei ZHANG , Wei LIU , Xiaxia LIANG , Huiyun CHANG
IPC分类号： A61K39/12 , C07K14/005 , A61K39/295 , A61P31/20
CPC分类号： A61K39/12 , C07K14/005 , A61K39/295 , A61P31/20 , C12N2710/12034 , C12N2710/12022 , A61K2039/552
摘要： The present disclosure relates to a cocktail vaccine of a recombinant African swine fever virus (ASFV) antigen and use thereof, and belongs to the field of biotechnology pharmacy. The cocktail vaccine is prepared by combination of recombinant proteins p30-modified p54, p72 epitope-N-segment amino acid sequence of pE248R, and N-terminal amino acid sequence of CD2v-C-terminal amino acid sequence of pEP153R with an adjuvant. The present disclosure provides a method for expressing and purifying the three recombinant proteins, as well as a ratio of each recombinant protein and the adjuvant for preparing the cocktail vaccine.
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公开(公告)号：US10766949B2
公开(公告)日：2020-09-08
申请号：US16540326
申请日：2019-08-14
发明人： Kun Li , Zengjun Lu , Zaixin Liu , Yimei Cao , Huifang Bao , Yingli Chen , Sheng Wang , Pinghua Li , Pu Sun , Xingwen Bai , Dong Li
IPC分类号： C07K16/10
摘要： The present invention relates to a method for preparing a whole bovine-derived broadly neutralizing antibody against serotype O foot-and-mouth disease virus, and belongs to the technical field of antibody preparation. The method includes the following steps: 1) conducting the immunization on the cattle; 2) screening for the serotype O foot-and-mouth disease virus antigen-specific single B cells; 3) amplifying variable region genes of the heavy and light chains; 4) acquiring constant region sequences of the heavy and light chains; 5) preparing a full-length heavy chain vector; 6) co-transfecting a cell, taking the supernatant of cell culture, and purifying. The method utilizes different foot-and-mouth disease virus strains to infect cattle and obtains a neutralizing antibody capable of neutralizing three topotypes of serotype O FMDVs by screening, and thus can obtain a whole bovine-derived broadly neutralizing monoclonal antibody.
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公开(公告)号：US10532077B2
公开(公告)日：2020-01-14
申请号：US16413598
申请日：2019-05-15
发明人： Huichen Guo , Shiqi Sun , Shichong Han , Hu Dong , Xiaoran Guo , Hong Yin , Jianxun Luo
IPC分类号： C07K14/005 , C07K16/10 , C12N7/00 , A61K39/00 , A61K39/12 , A61K35/76 , C12N15/86 , A61P31/14 , A61K39/39
摘要： A virus-like particle of Senecavirus A, the particle including a structural protein VP0, a structural protein VP1 and a structural protein VP3. The structural protein VP0 is encoded by a gene sequence represented by SEQ ID NO: 1. The structural protein VP1 is encoded by a gene sequence represented by SEQ ID NO: 2. The structural protein VP3 is encoded by a gene sequence represented by SEQ ID NO: 3.
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公开(公告)号：US11987797B2
公开(公告)日：2024-05-21
申请号：US18057588
申请日：2022-11-21
发明人： Haixue Zheng , Keshan Zhang , Ting Zhang , Tao Feng , Bo Yang , Xing Yang , Xijuan Shi , Hong Tian , Yi Ru , Fan Yang , Zixiang Zhu , Jianhong Guo , Jijun He , Xiangtao Liu
CPC分类号： C12N15/65 , C12N7/00 , C12N15/63 , C12N2710/12021 , C12N2710/12022
摘要： The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-β and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.
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8.发明公开公开(公告)号：US20230295641A1
公开(公告)日：2023-09-21
申请号：US18057588
申请日：2022-11-21
发明人： Haixue ZHENG , Keshan ZHANG , Ting ZHANG , Tao FENG , Bo YANG , Xing YANG , Xijuan SHI , Hong TIAN , Yi RU , Fan YANG , Zixiang ZHU , Jianhong GUO , Jijun HE , Xiangtao LIU
CPC分类号： C12N15/65 , C12N15/63 , C12N7/00 , C12N2710/12021 , C12N2710/12022
摘要： The present disclosure belongs to the technical field of biology, and in particular relates to an attenuated strain of African swine fever virus (ASFV) with IPTG-induced deletion of a D1133L gene and use thereof. In the present disclosure, it is firstly found that the D1133L protein of ASFV can inhibit production of IFN-β and downstream cytokines ISG-15 and ISG-56, and can be used as an immunosuppressant with a relatively strong immunosuppressive effect. In addition, the attenuated strain of ASFV with IPTG-induced deletion of the D1133L gene is constructed. Specifically, a screening expression cassette is inserted into a position before the non-structural protein gene D1133L of the ASFV using an Escherichia coli lac operator-repressor system, to obtain a recombinant virus. In the presence of the IPTG, the recombinant virus has similar characteristics to a wild-type virus; and in the absence of the IPTG, the expression of the D1133L protein is inhibited.
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9.发明申请公开(公告)号：US20220233671A1
公开(公告)日：2022-07-28
申请号：US17335573
申请日：2021-06-01
发明人： Yuefeng CHU , Shengli CHEN , Huafang HAO , Xinmin YAN , Lina MA , Yongsheng LIU
摘要： The present disclosure provides a subunit vaccine of contagious caprine pleuropneumonia and preparation method and use thereof, belonging to the technical field of preparation of animal infectious disease vaccine. The combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins comprises Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E; the mass ratio of Mycoplasma capricolum subsp. capripneumoniae immunoproteins A, B, C, D and E is (0.5-1.5): (0.5-1.5): (0.5-1.5): (0.5-1.5): (0.5-1.5). The subunit vaccine of contagious caprine pleuropneumonia comprises the combination of Mycoplasma capricolum subsp. capripneumoniae immunoproteins and adjuvant; the subunit vaccine has the advantages of high safety, good immunization effect, high stability and minor adverse effects.
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公开(公告)号：US20220031832A1
公开(公告)日：2022-02-03
申请号：US17328135
申请日：2021-05-24
发明人： Haixue Zheng , Fan Yang , Zixiang Zhu , Weijun Cao , Hong Tian , Keshan Zhang , Ting Wei , Min Zheng , Wei Zhang , Wen Dang , Xusheng Ma , Dan Li , Yi Ru , Jijun He , Jianhong Guo , Xiangtao Liu
IPC分类号： A61K39/125 , C12N15/10 , C12N15/66
摘要： The disclosure provides a recombinant nucleic acid of Seneca valley virus, a recombinant vaccine strain and preparation method and use thereof, and relates to the technical field of genetic engineering. The disclosure provides the recombinant nucleic acid of Seneca valley virus, recombinant Seneca valley virus comprising the recombinant nucleic acid, recombinant Seneca valley virus encoded by the recombinant nucleic acid, recombinant Seneca valley virus vaccine strain comprising the recombinant Seneca valley virus and preparation method and use thereof. According to the disclosure, a vaccine strain characterized by high antigen production capacity, remarkably reduced pathogenicity (even having no pathogenicity to pigs), strong antibody induction activity, high immune protection rate is prepared. The vaccine strain remarkably improves the biological safety and can be used for preventing and controlling Seneca valley virus in China and the neighboring countries.
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