公开(公告)号：US20220002802A1

公开(公告)日：2022-01-06

申请号：US17250408

申请日：2019-07-18

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN ,  Chenchen YANG

IPC: C12Q1/6881 ,  C12N15/10

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20230131285A1

公开(公告)日：2023-04-27

申请号：US17889654

申请日：2022-08-17

Applicant: Life Technologies Corporation

Inventor： Shrutii SARDA ,  Geoffrey LOWMAN

IPC: C12Q1/6883

Abstract: Prediction of a clinical response to a therapy of a subject with an autoimmune disease based on B cell immune repertoire may include determining a plurality of clone frequencies in a biological sample from the subject, wherein the clone frequencies include: IgM, IgD, IgG3, IgG4, IgA, IgM with first somatic hypermutation (SHM) level, IgG1 with second SHM level. A plurality of decision criteria may be applied to features including the plurality of clone frequencies. Each decision criterion applies at least one threshold to a feature, wherein the plurality of decision criteria provides a plurality of output values. The plurality of output values may be summed and a sigmoid transformation may be applied to the summed value to form a prediction value. The prediction value may be compared to a final threshold to identify the subject as a likely responder or non-responder to an autoimmune disease therapy.

公开(公告)号：US20220073983A1

公开(公告)日：2022-03-10

申请号：US17250406

申请日：2019-07-18

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Chenchen YANG ,  Geoffrey LOWMAN

IPC: C12Q1/6881 ,  C12N15/10

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of nucleic acid sequences of murine T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20210164047A1

公开(公告)日：2021-06-03

申请号：US17248722

申请日：2021-02-04

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C40B50/06

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20190085374A1

公开(公告)日：2019-03-21

申请号：US16120045

申请日：2018-08-31

Applicant: Life Technologies Corporation

Inventor： Geoffrey LOWMAN ,  Timothy LOONEY ,  Lifeng LIN ,  Elizabeth LINCH ,  Lauren MILLER

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20210285027A1

公开(公告)日：2021-09-16

申请号：US17302638

申请日：2021-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Geoffrey LOWMAN ,  Timothy LOONEY ,  Lifeng LIN ,  Elizabeth LINCH ,  Lauren MILLER

IPC: C12Q1/686 ,  C40B50/06 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20180208984A1

公开(公告)日：2018-07-26

申请号：US15873862

申请日：2018-01-17

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.