公开(公告)号：US20220002802A1

公开(公告)日：2022-01-06

申请号：US17250408

申请日：2019-07-18

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN ,  Chenchen YANG

IPC: C12Q1/6881 ,  C12N15/10

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20210285027A1

公开(公告)日：2021-09-16

申请号：US17302638

申请日：2021-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Geoffrey LOWMAN ,  Timothy LOONEY ,  Lifeng LIN ,  Elizabeth LINCH ,  Lauren MILLER

IPC: C12Q1/686 ,  C40B50/06 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20180208984A1

公开(公告)日：2018-07-26

申请号：US15873862

申请日：2018-01-17

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20210164047A1

公开(公告)日：2021-06-03

申请号：US17248722

申请日：2021-02-04

Applicant: Life Technologies Corporation

Inventor： Timothy LOONEY ,  Geoffrey LOWMAN ,  Lifeng LIN

IPC: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6844 ,  C12Q1/6811 ,  C12Q1/6881 ,  C12Q1/6883 ,  C40B50/06

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Variable regions associated with the immune cell receptor are resolved to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.

公开(公告)号：US20190085374A1

公开(公告)日：2019-03-21

申请号：US16120045

申请日：2018-08-31

Applicant: Life Technologies Corporation

Inventor： Geoffrey LOWMAN ,  Timothy LOONEY ,  Lifeng LIN ,  Elizabeth LINCH ,  Lauren MILLER

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/00

Abstract: The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire using genomic DNA from a biological sample. In one aspect, target-specific primer panels provide for the effective amplification of sequences of T cell receptor and/or B cell receptor chains with improved sequencing accuracy and resolution over the repertoire. Nucleic acid sequences of variable regions associated with the immune cell receptor are determined to effectively portray clonal diversity of a biological sample and/or differences associated with the immune cell repertoire of a biological sample.