公开(公告)号：US20220205033A1

公开(公告)日：2022-06-30

申请号：US17553295

申请日：2021-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Joseph PODHASKY ,  Shanti SHANKAR

IPC: C12Q1/6869 ,  C12Q1/6806 ,  G16B50/20

Abstract: A method for determining a sequence of nucleic acids includes purifying the nucleic acids from a sample with a purification instrument in accordance with a purification plan of a run plan. The purified nucleic acids are disposed in a transfer plate. Disposition of the purified nucleic acids is stored in a transfer file. The method further includes transferring the transfer plate to a sequencing instrument; transferring the transfer file to the sequencing instrument; and sequencing the nucleic acids with the sequencing instrument in accordance with a sequencing plan of the run plan and based on the transfer file.

公开(公告)号：US20220176369A1

公开(公告)日：2022-06-09

申请号：US17526979

申请日：2021-11-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph PODHASKY ,  Mark Reed ,  Nathan Kane

IPC: B01L3/02 ,  B01L3/00

Abstract: A system includes a pipetting system including a 3-axis gantry; a sled mechanism to select a magnetic comb from a set of magnetic combs; a fluorometer; and a set of receptacles to receive welled plates. A method for purifying nucleic acids includes applying a sample to a well of a multi-well plate, selecting a magnetic comb from a set of magnetic combs disposed on a gantry system, collecting magnetic beads using the magnetic comb, collecting nucleic acid using the magnetic beads, and eluting the nucleic acid from the beads.

公开(公告)号：US20220170093A1

公开(公告)日：2022-06-02

申请号：US17527997

申请日：2021-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph PODHASKY ,  Mark REED ,  David MARRAN ,  Chiu Tai Andrew WONG ,  Angie CHENG ,  Ru CAO

IPC: C12Q1/6874 ,  B01J19/00

Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a total instrument run time in a range of 16 hours to 22 hours to determine variant calls when sequencing 6 cfTNA extracted polynucleotide samples using a targeted assay with one nucleic acid pool per sample.