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    SYSTEM AND METHOD FOR PREPARING A SEQUENCING DEVICE
    1.
    发明申请
    SYSTEM AND METHOD FOR PREPARING A SEQUENCING DEVICE 审中-公开

    公开(公告)号：US20200072826A1

    公开(公告)日：2020-03-05

    申请号：US16543389

    申请日：2019-08-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Hua YU ,  Xiaoling YANG ,  Rui ZHENG ,  Chiu Tai Andrew WONG ,  Jeremy GRAY

    IPC: G01N33/543 ,  C12Q1/6844

    Abstract: The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to mixing magnetic beads with sequencing beads to form a solution. The solution containing both beads is injected onto a microchip having a plurality of microwells. The magnetic beads may have larger diameter than the microwell while the sequencing beads may have a smaller diameter, allowing them to enter and reside in the microwell. One or more magnets positioned under the microchip move back and forth across the microchip surface. The magnetic beads form a line and follow the movement of the magnets. During rounds of sweeping, the sequencing beads load into the respective wells. The magnets may be disengaged and the magnetic beads may be washed away after the sequencing beads are loaded.

    SYSTEM AND METHOD FOR AUTOMATED REPEAT SEQUENCING
    2.
    发明申请
    SYSTEM AND METHOD FOR AUTOMATED REPEAT SEQUENCING 有权

    公开(公告)号：US20220154176A1

    公开(公告)日：2022-05-19

    申请号：US17525737

    申请日：2021-11-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Kylan SZETO ,  Shanti SHANKAR ,  Mark BEAUCHEMIN

    IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/6825 ,  G01N35/10

    Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS
    4.
    发明申请
    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS 审中-公开

    公开(公告)号：US20200041444A1

    公开(公告)日：2020-02-06

    申请号：US16362367

    申请日：2019-03-22

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

    IPC: G01N27/414 ,  G16B30/00

    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS
    6.
    发明申请
    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS 有权

    公开(公告)号：US20220146450A1

    公开(公告)日：2022-05-12

    申请号：US17499676

    申请日：2021-10-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

    IPC: G01N27/414 ,  G16B30/00

    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

    SYSTEM AND METHOD FOR SEQUENCING
    7.
    发明申请
    SYSTEM AND METHOD FOR SEQUENCING 有权

    公开(公告)号：US20210054450A1

    公开(公告)日：2021-02-25

    申请号：US16994318

    申请日：2020-08-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark REED ,  Chiu Tai Andrew WONG ,  David MARRAN

    IPC: C12Q1/6874

    Abstract: A sequencing system includes an automated sequencing instrument adapted to determine variant calls for one or more extracted polynucleotide samples with a performance of at least 98.5% raw read accuracy and a run time in a range of 5 hours to 14 hours to determine variant calls for 4 extracted polynucleotide samples using a targeted assay with one DNA pool per sample and an average amplicon size in a range of 100 to 120 bases.

    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS
    9.
    发明申请
    APPARATUSES, METHODS, SYSTEMS, AND COMPUTER-READABLE MEDIA FOR FLUID POTENTIAL ARTIFACT CORRECTION IN REAGENT DELIVERY SYSTEMS 有权
    设备，方法，系统和计算机可读介质，用于在试剂递送系统中进行流体潜在的矫正校正

    公开(公告)号：US20160103093A1

    公开(公告)日：2016-04-14

    申请号：US14853026

    申请日：2015-09-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Chiu Tai Andrew WONG ,  Todd REARICK ,  John DONOHUE

    IPC: G01N27/414 ,  G06F19/22 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  G16B30/00

    Abstract: A method for correcting nucleotide incorporation signals for fluid potential effects or disturbances arising in nucleic acid sequencing-by-synthesis includes: disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, the template polynucleotide strands having a sequencing primer and a polymerase bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed through a fluid manifold, the fluid manifold comprising passages for flowing nucleotide species and a branch passage for flowing a solution, the branch passage comprising a reference electrode and a sensing electrode; obtaining a plurality of nucleotide incorporation signals corresponding to the plurality of defined spaces, the nucleotide incorporation signals having a signal intensity related to a number of nucleotide incorporations; and correcting at least some of the plurality of nucleotide incorporation signals for fluid potential effects or disturbances.

    Abstract translation: 用于校正核酸并入信号的方法，用于在核酸序列合成中产生的流体潜在效应或紊乱，包括：将多个模板多核苷酸链设置在设置在传感器阵列上的多个限定的空间中，所述模板多核苷酸链具有测序 引物和与之结合的聚合酶; 将模板多核苷酸链暴露于流过流体歧管的一系列核苷酸物质流，该流体歧管包括用于流动核苷酸物质的通道和用于使溶液流动的分支通道，该分支通道包括参考电极和感测电极; 获得对应于所述多个限定空间的多个核苷酸掺入信号，所述核苷酸掺入信号具有与多个核苷酸结合相关的信号强度; 以及校正所述多个核苷酸并入信号中的至少一些用于流体潜在效应或干扰。

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