公开(公告)号：US20220205033A1

公开(公告)日：2022-06-30

申请号：US17553295

申请日：2021-12-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark REED ,  Joseph PODHASKY ,  Shanti SHANKAR

IPC: C12Q1/6869 ,  C12Q1/6806 ,  G16B50/20

Abstract: A method for determining a sequence of nucleic acids includes purifying the nucleic acids from a sample with a purification instrument in accordance with a purification plan of a run plan. The purified nucleic acids are disposed in a transfer plate. Disposition of the purified nucleic acids is stored in a transfer file. The method further includes transferring the transfer plate to a sequencing instrument; transferring the transfer file to the sequencing instrument; and sequencing the nucleic acids with the sequencing instrument in accordance with a sequencing plan of the run plan and based on the transfer file.

公开(公告)号：US20220154176A1

公开(公告)日：2022-05-19

申请号：US17525737

申请日：2021-11-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chiu Tai Andrew WONG ,  Kylan SZETO ,  Shanti SHANKAR ,  Mark BEAUCHEMIN

IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/6825 ,  G01N35/10

Abstract: A method for sequencing a target polynucleotide includes detecting a first series of nucleotide incorporations complementary to at least a portion of the target polynucleotide. The first series of nucleotide incorporations forms a first complementary polynucleotide. The target nucleotide is secured to a substrate disposed in a sequencing zone of an assembly. The method further includes moving the substrate to which the target nucleotide is secured to a templating zone of the assembly; removing the first complementary polynucleotide when the substrate is disposed at the templating zone of the assembly, the target polynucleotide remaining secured to the substrate; following the removing, moving the substrate to which the target polynucleotide is secured to the sequencing zone; and detecting a second series of nucleotide incorporations complementary to at least a portion of the target polynucleotide, the second series of nucleotide incorporations forming a second complementary polynucleotide.

公开(公告)号：US20200075130A1

公开(公告)日：2020-03-05

申请号：US16543475

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shanti SHANKAR ,  Sean ZIMMERMAN ,  Mark BEAUCHEMIN ,  John THOMPSON

IPC: G16B45/00 ,  G06T1/00 ,  G16B30/10 ,  G01N1/28

Abstract: A method for user guided initiating of an instrument includes receiving a run plan via a user interface of the instrument; indicating on the user interface, based on the run plan, a consumable to be provided to the instrument; detecting the presence of the consumable using a vision system; and indicating the presence of the consumable via the user interface.