公开(公告)号：US20210277448A1

公开(公告)日：2021-09-09

申请号：US17301040

申请日：2021-03-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/686 ,  C12Q1/6844

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.

公开(公告)号：US20240376534A1

公开(公告)日：2024-11-14

申请号：US18689573

申请日：2022-09-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kalpith RAMAMOORTHI ,  Noah ELDER

IPC: C12Q1/686

Abstract: Disclosed are master mix compositions, kits, and related methods for use in amplifying a target nucleic acid. A master mix composition may be formulated to enable effective reaction mixture loading onto a dPCR plate, provide high proportion of valid/readable partitions, enable identification and distinguishing between amplified and unamplified partitions, enable detection of low abundance targets amidst high background levels of non-target nucleic acids and/or amidst inhibiting agents, and/or enable effective detection of low frequency mutant alleles.

公开(公告)号：US20170292144A1

公开(公告)日：2017-10-12

申请号：US15481021

申请日：2017-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01D19/0404 ,  B01D19/0409 ,  B01D19/0413 ,  B01D19/0418 ,  B01D19/0422 ,  C12Q1/6846 ,  C12Q2525/125

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.