公开(公告)号：US08906628B2

公开(公告)日：2014-12-09

申请号：US13870779

申请日：2013-04-25

Applicant: Life Technologies Corporation

Inventor： Paolo Vatta ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而不是大肠杆菌O55：H7的方法和试剂盒，来自样品如：复合食物基质，水，饮料，发酵液，法医和生物样品以及包括食物在内的环境样品 加工和制造表面。 在一些实施方案中，本公开的方法包括：使至少第一对多核苷酸引物与至少第一靶多核苷酸序列杂交，将至少第二对多核苷酸引物与至少第二靶多核苷酸序列杂交， 至少第一和至少第二靶多核苷酸序列，以及检测第一和第二扩增的靶多核苷酸序列产物，其中第一扩增的目标多核苷酸序列产物和第二扩增的靶多核苷酸序列产物的检测指示E. 大肠杆菌O157：H7在样品中，而不是大肠杆菌O55：H7。

公开(公告)号：US20130236896A1

公开(公告)日：2013-09-12

申请号：US13870779

申请日：2013-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Paolo VATTA ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而非大肠杆菌O55：H7的分析，方法和试剂盒，来自复杂的食物基质，水，饮料样品，发酵液，法医样品，环境样品（例如 ，土壤，污垢，垃圾，污水，空气或水），包括食品加工和制造表面，或生物样品。