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    Analysis of Y-chromosome STR markers
    1.
    发明授权
    Analysis of Y-chromosome STR markers 有权

    公开(公告)号：US11453917B2

    公开(公告)日：2022-09-27

    申请号：US16840168

    申请日：2020-04-03

    Applicant: Life Technologies Corporation

    Inventor： Rixun Fang ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

    IPC: C12Q1/6888

    Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

    Multi-primer Assay for Mycoplasma Detection
    2.
    发明申请
    Multi-primer Assay for Mycoplasma Detection 有权
    用于支原体检测的多引物测定

    公开(公告)号：US20140378330A1

    公开(公告)日：2014-12-25

    申请号：US14314985

    申请日：2014-06-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Olga PETRAUSKENE ,  Pius Brzoska ,  Somaya Bit ,  Jen-Kuei Liu ,  Robert Tebbs ,  Manohar Furtado

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: Disclosed is a multi-primer amplification assay, method and kits for detecting Mycoplasma species and closely related species utilizing a plurality of oligonucleotide primers in contact with a sample in a single vessel and detecting the amplification product, wherein the presence of an amplification product indicates Mycoplasma in the sample.

    Abstract translation: 公开了一种多引物扩增测定法，用于利用在单个容器中与样品接触的多个寡核苷酸引物检测支原体物种和密切相关物种的检测扩增产物的方法和试剂盒，其中扩增产物的存在表示支原体 在样品中。

    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS
    3.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF MULTIPLE MICROORGANISMS 审中-公开
    用于检测多种微生物的组合物和方法

    公开(公告)号：US20140005061A1

    公开(公告)日：2014-01-02

    申请号：US13790447

    申请日：2013-03-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Robert TEBBS ,  Craig Cummings ,  Pius Brzoska ,  Sharon Matheny ,  Catherine O'Connell ,  Manohar Furtado ,  Nikolay Sergeev ,  Maxim Brevnov

    IPC: C12Q1/68

    CPC classification number: C12Q1/689 ,  C12Q2600/16

    Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

    Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

    ANALYSIS OF Y-CHROMOSOME STR MARKERS
    6.
    发明申请
    ANALYSIS OF Y-CHROMOSOME STR MARKERS 审中-公开

    公开(公告)号：US20200299786A1

    公开(公告)日：2020-09-24

    申请号：US16840168

    申请日：2020-04-03

    Applicant: Life Technologies Corporation

    Inventor： Rixun Fang ,  Manohar Furtado ,  Manfred Kayser ,  Kaye Ballantyne

    IPC: C12Q1/6888

    Abstract: The methods and compositions provided herein relate to the discovery of 13 STR markers, found on the human Y chromosome, having surprisingly high mutation rates when compared with 173 other Y-STR markers known today. The set of RM-Y-STRs may overcome the current dilemma of Y-chromosome analysis in forensic applications due to their extraordinary mutation properties. Embodiments of the invention include methods for allelic determination of rapidly-mutating Y-STR markers, amplification primers for the analysis of rapidly-mutating Y-STR markers, allelic ladders for analysis of rapidly-mutating Y-STR markers, and kits for the analysis of rapidly-mutating Y-STR markers.

    Nucleic acid amplification methods for specific detection of E. coli O157:H7 without co-detection of E. coli O55:H7
    8.
    发明授权
    Nucleic acid amplification methods for specific detection of E. coli O157:H7 without co-detection of E. coli O55:H7 有权
    用于特异性检测大肠杆菌O157：H7而不共同检测大肠杆菌O55：H7的核酸扩增方法

    公开(公告)号：US08906628B2

    公开(公告)日：2014-12-09

    申请号：US13870779

    申请日：2013-04-25

    Applicant: Life Technologies Corporation

    Inventor： Paolo Vatta ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

    IPC: C12Q1/68 ,  C12P19/34

    CPC classification number: C12Q1/689 ,  C12Q2600/142

    Abstract: Disclosed are methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.

    Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而不是大肠杆菌O55：H7的方法和试剂盒，来自样品如：复合食物基质，水，饮料，发酵液，法医和生物样品以及包括食物在内的环境样品 加工和制造表面。 在一些实施方案中，本公开的方法包括：使至少第一对多核苷酸引物与至少第一靶多核苷酸序列杂交，将至少第二对多核苷酸引物与至少第二靶多核苷酸序列杂交， 至少第一和至少第二靶多核苷酸序列，以及检测第一和第二扩增的靶多核苷酸序列产物，其中第一扩增的目标多核苷酸序列产物和第二扩增的靶多核苷酸序列产物的检测指示E. 大肠杆菌O157：H7在样品中，而不是大肠杆菌O55：H7。

    Methods and kits for extraction of DNA
    10.
    发明授权
    Methods and kits for extraction of DNA 有权

    公开(公告)号：US09422544B2

    公开(公告)日：2016-08-23

    申请号：US13931110

    申请日：2013-06-28

    Applicant: Life Technologies Corporation

    Inventor： Maxim Brevnov ,  Hemant Pawar ,  Manohar Furtado ,  Jaiprakash Shewale

    IPC: C07H21/02 ,  C12Q1/68 ,  C12N15/10

    CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806 ,  C12Q2563/143 ,  C12Q2523/308

    Abstract: Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

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