公开(公告)号：US20160186242A1

公开(公告)日：2016-06-30

申请号：US14978243

申请日：2015-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20130260374A1

公开(公告)日：2013-10-03

申请号：US13785409

申请日：2013-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei JI ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

公开(公告)号：US10513741B2

公开(公告)日：2019-12-24

申请号：US15891454

申请日：2018-02-08

Applicant: Life Technologies Corporation

Inventor： Yongmei (Alexis) Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/689 ,  C12Q1/6806

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US09546405B2

公开(公告)日：2017-01-17

申请号：US14309865

申请日：2014-06-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Olga Petrauskene ,  Craig Cummings ,  Paolo Vatta ,  Robert Tebbs ,  Priya Balachandran ,  Patrick Zoder ,  Lily Wong

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

Abstract translation: 本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。 本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。 一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏，（L.marthii）（以前称为incertae-sedis），伊卡诺菌（L. ivanovii）和L. 还描述了用于检测李斯特菌的试剂盒。 在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。 在一些实施方案中，0.2-2cfu的李斯特菌属 在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

公开(公告)号：US20140005061A1

公开(公告)日：2014-01-02

申请号：US13790447

申请日：2013-03-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert TEBBS ,  Craig Cummings ,  Pius Brzoska ,  Sharon Matheny ,  Catherine O'Connell ,  Manohar Furtado ,  Nikolay Sergeev ,  Maxim Brevnov

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/16

Abstract: The present disclosure describes compositions, methods and kits for detection of one or multiple microorganism contaminants in samples. Some embodiments relate to detecting one or more microorganisms producing virulence factors such as shiga toxin stx1 and stx2 and eae. Some embodiments relate to detection of STEC microorganisms including an E. coli O26, an E. coli O45, an E. coli O103, an E. coli O111, an E. coli O121 or an E. coli O145. In some embodiments, compositions, methods and kits can detect and identify individual serotypes of shiga toxin producing microorganisms. Workflows for multiple microbe detection and identification are also described.

Abstract translation: 本公开描述了用于检测样品中一种或多种微生物污染物的组合物，方法和试剂盒。 一些实施方案涉及检测产生毒力因子的一种或多种微生物，例如志贺毒素stx1和stx2和eae。 一些实施方案涉及检测STEC微生物，包括大肠杆菌O26，大肠杆菌O45，大肠杆菌O103，大肠杆菌O111，大肠杆菌O121或大肠杆菌O145。 在一些实施方案中，组合物，方法和试剂盒可以检测和鉴定产生志贺毒素的微生物的个体血清型。 还描述了用于多个微生物检测和鉴定的工作流程。

公开(公告)号：US09255299B2

公开(公告)日：2016-02-09

申请号：US13785409

申请日：2013-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Yongmei Ji ,  Pius Brzoska ,  Angela Burrell ,  Craig Cummings ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6806 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of Mycobacterium avium subsp. paratuberculosis from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性和差异性检测鸟分枝杆菌亚种的组合物，测定法，方法，诊断方法，试剂盒和诊断试剂盒。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（例如土壤，污垢，垃圾，污水，空气或水）的样品中的副结核病，包括食品加工和制造表面或生物样品。

公开(公告)号：US20150045242A1

公开(公告)日：2015-02-12

申请号：US14309865

申请日：2014-06-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Olga PETRAUSKENE ,  Craig Cummings ,  Paolo Vatta ,  Robert Tebbs ,  Priya Balachandran ,  Patrick Zoder ,  Lily Wong

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several Listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of Listeria from each other, for example L. grayi may be detected independently of other Listeria spp. Some embodiments also describe a duplexed assay that can detect L. monocytogenes, L. innocua, L. welshimeri, L. seelgeri, L. marthii (formerly incertae-sedis), L. ivanovii, and L. grayi. Kits for detection of Listeria are also described. In some embodiments, methods and kits of the disclosure may comprise a TAQMAN® assay. In some embodiments, 0.2-2 cfu of Listeria spp. are detected using the compositions, methods and kits after a 24-28 hour enrichment period.

Abstract translation: 本公开的实施方案涉及分离的核酸序列，其使用方法和用于检测样品中的几种利斯特氏菌物种的工作流程，特别是在食品或环境样品中。 本公开的实施方案还可以用于彼此检测李斯特氏菌的一种或多种物种或菌株，例如，可以独立于其他李斯特菌属物种检测L.crilli。 一些实施方案还描述了可以检测单核细胞增生李斯特氏菌（L. innocua），威尔逊氏乳杆菌（L.welshimeri），塞贝尔氏球菌（L.ceelgeri），马氏，（L.marthii）（以前称为incertae-sedis），伊卡诺菌（L. ivanovii）和L. 还描述了用于检测李斯特菌的试剂盒。 在一些实施方案中，本公开的方法和试剂盒可以包括TAQMAN测定。 在一些实施方案中，0.2-2cfu的李斯特菌属 在24-28小时浓缩期后使用组合物，方法和试剂盒检测。

公开(公告)号：US08906628B2

公开(公告)日：2014-12-09

申请号：US13870779

申请日：2013-04-25

Applicant: Life Technologies Corporation

Inventor： Paolo Vatta ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from samples such as: complex food matrices, water, beverages, fermentation broths, forensic & biological samples, and environmental samples including food processing and manufacturing surfaces. In some embodiments, a method of the disclosure comprises: hybridizing at least a first pair of polynucleotide primers to at least a first target polynucleotide sequence, hybridizing at least a second pair of polynucleotide primers to at least a second target polynucleotide sequence, amplifying the at least first and at least second target polynucleotide sequences, and detecting the first and second amplified target polynucleotide sequence products, wherein the detection of both the first amplified target polynucleotide sequence product and the second amplified target polynucleotide sequence product is indicative of the presence of E. coli O157:H7 in a sample and not E. coli O55:H7.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而不是大肠杆菌O55：H7的方法和试剂盒，来自样品如：复合食物基质，水，饮料，发酵液，法医和生物样品以及包括食物在内的环境样品 加工和制造表面。 在一些实施方案中，本公开的方法包括：使至少第一对多核苷酸引物与至少第一靶多核苷酸序列杂交，将至少第二对多核苷酸引物与至少第二靶多核苷酸序列杂交， 至少第一和至少第二靶多核苷酸序列，以及检测第一和第二扩增的靶多核苷酸序列产物，其中第一扩增的目标多核苷酸序列产物和第二扩增的靶多核苷酸序列产物的检测指示E. 大肠杆菌O157：H7在样品中，而不是大肠杆菌O55：H7。

公开(公告)号：US09334542B2

公开(公告)日：2016-05-10

申请号：US13785531

申请日：2013-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Craig Cummings ,  Pius Brzoska ,  Angela Burrell ,  Catherine O'Connell

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/112

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.

Abstract translation: 公开了用于特异性和差异性检测非分枝杆菌鸟属亚种的组合物，测定，方法，诊断方法，试剂盒和诊断试剂盒。 副结核病（非MAP）生物体，其中非MAP生物体是不属于鸟分枝杆菌亚种的鸟分枝杆菌复合体（MAC）生物。 来自包括兽医样品，临床样品，食品样品，法医样品，环境样品（如土壤，污垢，垃圾，污水，空气或水）在内的样品的副结核病（MAP）生物，包括食品加工和制造表面，或 生物样品。 示例性非MAP生物体，包括鸟分枝杆菌 鸟类（MAA），鸟分枝杆菌 山楂（MAH）和鸟分枝杆菌 可以通过本发明的组合物，试剂盒和方法检测silvaticum（MAS）。

公开(公告)号：US20130236896A1

公开(公告)日：2013-09-12

申请号：US13870779

申请日：2013-04-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Paolo VATTA ,  Olga Petrauskene ,  Manohar Furtado ,  Pius Brzoska ,  Lily Wong ,  Melissa Barker ,  Craig Cummings

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q2600/142

Abstract: Disclosed are assays, methods and kits for the specific detection of E. coli O157:H7 and not E. coli O55:H7 from complex food matrices, water, a beverage sample, a fermentation broth, a forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

Abstract translation: 公开了用于特异性检测大肠杆菌O157：H7而非大肠杆菌O55：H7的分析，方法和试剂盒，来自复杂的食物基质，水，饮料样品，发酵液，法医样品，环境样品（例如 ，土壤，污垢，垃圾，污水，空气或水），包括食品加工和制造表面，或生物样品。