公开(公告)号：US20190309341A1

公开(公告)日：2019-10-10

申请号：US16306609

申请日：2017-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Wenjun ZHOU ,  Shih-Jung HUANG ,  Monica TOMASZEWSKI

IPC: C12Q1/37 ,  G01N33/58 ,  C07K5/113 ,  C07K5/103 ,  G01N21/64

Abstract: Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase