公开(公告)号：US20220372522A1

公开(公告)日：2022-11-24

申请号：US17766998

申请日：2020-10-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Pei-Zhong TANG ,  Robert POTTER

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/11 ,  C07K14/245

Abstract: The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.

公开(公告)号：US20200325471A1

公开(公告)日：2020-10-15

申请号：US16864445

申请日：2020-05-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Pei-Zhong TANG

IPC: C12N15/10 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/6855

Abstract: Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.

公开(公告)号：US20180016572A1

公开(公告)日：2018-01-18

申请号：US15646851

申请日：2017-07-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Pei-Zhong TANG

IPC: C12N15/10

Abstract: Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.