公开(公告)号：US20170306306A1

公开(公告)日：2017-10-26

申请号：US15520533

申请日：2015-10-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert POTTER ,  Jonathan CHESNUT ,  Xiquan LIANG

IPC: C12N9/22 ,  C12N15/90 ,  C12N2310/20

CPC classification number: C12N9/22 ,  C07K2319/00 ,  C07K2319/81 ,  C12N15/902 ,  C12N2310/20

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

公开(公告)号：US20200325514A1

公开(公告)日：2020-10-15

申请号：US16736258

申请日：2020-01-07

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Todd PETERSON ,  Axel TREFZER ,  Thomas POEHMERER ,  Federico KATZEN ,  Xiquan LIANG

IPC: C12P19/34 ,  C12N15/10

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20180195089A1

公开(公告)日：2018-07-12

申请号：US15842664

申请日：2017-12-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha RAVINDER ,  Korbinian HEIL ,  Yizhu GUO ,  Xiquan LIANG ,  Robert POTTER ,  Sanjay KUMAR

IPC: C12N15/90 ,  C12N15/10 ,  C12Q1/686 ,  C12P19/34 ,  C12N15/11

CPC classification number: C12N15/907 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/3519 ,  C12P19/34 ,  C12Q1/686 ,  C12Q2525/143

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US20160122792A1

公开(公告)日：2016-05-05

申请号：US14775648

申请日：2014-03-14

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Todd PETERSON ,  Axel TREFZER ,  Thomas POEHMERER ,  Federico KATZEN ,  Xiquan LIANG

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1031 ,  C12N15/1093

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20220033858A1

公开(公告)日：2022-02-03

申请号：US17327002

申请日：2021-05-21

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Namritha RAVINDER ,  Korbinian HEIL ,  Yizhu GUO ,  Xiquan LIANG ,  Robert POTTER ,  Sanjay KUMAR

IPC: C12N15/90 ,  C12Q1/686 ,  C12N15/10 ,  C12N15/11 ,  C12P19/34

Abstract: The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

公开(公告)号：US20210054415A1

公开(公告)日：2021-02-25

申请号：US17093486

申请日：2020-11-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xin YU ,  Xiquan LIANG ,  Xavier de MOLLERAT du JEU ,  Robert Jason POTTER

IPC: C12N15/90 ,  C12N9/22 ,  C12N15/88 ,  A61K48/00

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

公开(公告)号：US20170254775A1

公开(公告)日：2017-09-07

申请号：US15597928

申请日：2017-05-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John LEITE ,  Robert POPE ,  Mahbod HAJIVANDI ,  Xiquan LIANG

IPC: G01N27/62 ,  G01N33/60 ,  G01N33/68

Abstract: The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. Methods of the present invention also include methods for quantifying biomolecules where the copy biomolecule standard is made by expressing the biomolecule using a recombinant cell. Further included are the biomolecule standards themselves, method for making such standards, kits, systems, reagents, and engineered cells relating to the use of biomolecule standards in mass spectrometric analysis.

公开(公告)号：US20240247286A1

公开(公告)日：2024-07-25

申请号：US18374673

申请日：2023-09-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xiquan LIANG ,  Robert Jason POTTER ,  Lansha PENG

IPC: C12N15/90 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63

CPC classification number: C12N15/907 ,  C07K14/195 ,  C12N9/22 ,  C12N15/63 ,  C12N2800/80 ,  C12N2800/95 ,  C12N2810/40

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.

公开(公告)号：US20230151345A1

公开(公告)日：2023-05-18

申请号：US18071206

申请日：2022-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Robert POTTER ,  Jonathan CHESNUT ,  Xiquan LIANG

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.

公开(公告)号：US20200299729A1

公开(公告)日：2020-09-24

申请号：US16644445

申请日：2018-09-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chi-Hsuan CHANG ,  Xiquan LIANG

IPC: C12N15/87 ,  C12N15/11 ,  C12N9/22 ,  C12N5/0783

Abstract: The present disclosure relates to methods, kits, and compositions for improving the efficiency of homologous recombination. In particular, the disclosure relates to methods for introducing a nucleic acid cutting entity for DNA editing into cells that are difficult to transfect. Generally, the nucleic acid cutting entity is introduced into the cell without the use of viral vectors. The disclosure also relates to cloning DNA molecules directly into a genome with the combined use of promoter trapping and short homology arms, nuclear localization signal, and/or binding one or more DNA binding agents (TAL effector domain or truncated guide RNA bound by Cas9) to specific sites thereby displacing or restructuring chromatin at the target locus, and/or it increasing the accessibility of the target locus to further enzymatic modifications. The methods and compositions provided herein are, inter alia, useful for genome editing and enhancing enzymatic processes involved therein.