公开(公告)号：US08969100B2

公开(公告)日：2015-03-03

申请号：US13379320

申请日：2010-03-11

申请人： Makoto Hikage ,  Kohsuke Gonda ,  Motohiro Takeda ,  Takashi Kamei ,  Noriaki Ohuchi ,  Hideki Gouda ,  Yasushi Nakano

发明人： Makoto Hikage ,  Kohsuke Gonda ,  Motohiro Takeda ,  Takashi Kamei ,  Noriaki Ohuchi ,  Hideki Gouda ,  Yasushi Nakano

IPC分类号： G01N33/543 ,  G01N33/574 ,  G01N21/64 ,  A61K49/00 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/58 ,  A61B5/00

CPC分类号： G01N21/6458 ,  A61B5/0071 ,  A61B5/41 ,  A61B5/418 ,  A61K49/0017 ,  A61K49/0067 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/57492 ,  G01N33/588 ,  G01N2201/0221 ,  Y10T436/13

摘要： Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity.

摘要翻译： 在前哨淋巴结中以高精度检测转移性癌细胞可存在的区域。 将量子点注入生物体内的癌症附近，通过荧光鉴定前哨淋巴结的位置。 随后，提取前哨淋巴结。 对于注射量子点提取的前哨淋巴结，采用精细荧光测量法进行结构分析，采用共焦荧光显微镜进行单分子观察。 具体地，针对前哨淋巴结中的多个区域中的每个区域测量荧光强度，并且在测量的多个区域中，以荧光强度的降序将一个或多个区域作为传入淋巴管流入区域检测。

公开(公告)号：US20120107831A1

公开(公告)日：2012-05-03

申请号：US13379320

申请日：2010-03-11

申请人： Makoto Hikage ,  Kohsuke Gonda ,  Motohiro Takeda ,  Takashi Kamei ,  Noriaki Ohuchi ,  Hideki Gouda ,  Yasushi Nakano

发明人： Makoto Hikage ,  Kohsuke Gonda ,  Motohiro Takeda ,  Takashi Kamei ,  Noriaki Ohuchi ,  Hideki Gouda ,  Yasushi Nakano

IPC分类号： G01N21/64 ,  B82Y5/00

CPC分类号： G01N21/6458 ,  A61B5/0071 ,  A61B5/41 ,  A61B5/418 ,  A61K49/0017 ,  A61K49/0067 ,  B82Y5/00 ,  B82Y15/00 ,  G01N33/57492 ,  G01N33/588 ,  G01N2201/0221 ,  Y10T436/13

摘要： Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity.

摘要翻译： 在前哨淋巴结中以高精度检测转移性癌细胞可存在的区域。 将量子点注入生物体内的癌症附近，通过荧光鉴定前哨淋巴结的位置。 随后，提取前哨淋巴结。 对于注射量子点提取的前哨淋巴结，采用精细荧光测量法进行结构分析，采用共焦荧光显微镜进行单分子观察。 具体地，针对前哨淋巴结中的多个区域中的每个区域测量荧光强度，并且在测量的多个区域中，以荧光强度的降序将一个或多个区域作为传入淋巴管流入区域检测。