公开(公告)号：US09909109B2

公开(公告)日：2018-03-06

申请号：US14384232

申请日：2013-03-22

Applicant: Novozymes A/S

Inventor： Jakob Skjold-Joergensen ,  Jesper Vind ,  Allan Svendsen

IPC: C12N9/18 ,  C12N9/20 ,  C12N1/14 ,  C12N1/20 ,  C12N15/52 ,  C12N15/63 ,  C11D3/386

CPC classification number: C12N9/18 ,  C11D3/38627 ,  C12N1/14 ,  C12N1/20 ,  C12N9/20 ,  C12N15/52 ,  C12N15/63

Abstract: The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.

公开(公告)号：US20170314001A1

公开(公告)日：2017-11-02

申请号：US15531983

申请日：2015-12-01

Applicant: Novozymes A/S

Inventor： Allan Svendsen ,  Jesper Vind ,  Lone Baunsgaard ,  Jakob Skjold-Joergensen ,  Vikram Kjoeller Bhatia ,  Lars Henrik Oestergaard

IPC: C12N9/20 ,  C11D3/386

CPC classification number: C12N9/20 ,  C11D3/38627 ,  C12Y301/01 ,  C12Y301/01003

Abstract: The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, has lipase activity, comprises a substitution at a position corresponding to position 92 and/or 96; and remains unaltered at the positions corresponding to positions 231, 233 and 254 of SEQ ID NO:2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10457921B2

公开(公告)日：2019-10-29

申请号：US15531983

申请日：2015-12-01

Applicant: Novozymes A/S

Inventor： Allan Svendsen ,  Jesper Vind ,  Lone Baunsgaard ,  Jakob Skjold-Joergensen ,  Vikram Kjoeller Bhatia ,  Lars Henrik Oestergaard

IPC: C12N9/20 ,  C11D3/36 ,  C11D3/386

Abstract: The present invention relates to a variant of a parent lipase, wherein said variant has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, has lipase activity, comprises a substitution at a position corresponding to position 92 and/or 96; and remains unaltered at the positions corresponding to positions 231, 233 and 254 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20150031111A1

公开(公告)日：2015-01-29

申请号：US14384232

申请日：2013-03-22

Applicant: Novozymes A/S

Inventor： Jakob Skjold-Joergensen ,  Jesper Vind ,  Allan Svendsen

IPC: C12N9/18 ,  C11D3/386

CPC classification number: C12N9/18 ,  C11D3/38627 ,  C12N1/14 ,  C12N1/20 ,  C12N9/20 ,  C12N15/52 ,  C12N15/63

Abstract: The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.

Abstract translation: 本发明涉及分离的脂肪酶变体，其包含在对应于SEQ ID NO的成熟多肽的I86D，E，N，Q，E87C，I90D，E，Q和N92D，E，Q的一个或多个位置的取代 2，其中变体具有脂肪酶活性。 在一些实施方案中，本发明涉及编码变体的分离的多核苷酸; 核酸构建体，载体和包含所述多核苷酸的宿主细胞; 生产变体的方法; 和包含这些变体的组合物。 本发明还涉及获得脂肪酶变体的方法; 清洁方法; 并使用脂肪酶变体进行清洁。