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    Methods for producing lipases
    1.
    发明授权
    Methods for producing lipases 有权

    公开(公告)号：US11371029B2

    公开(公告)日：2022-06-28

    申请号：US16865744

    申请日：2020-05-04

    Applicant: Novozymes A/S

    Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

    IPC: C12N9/20

    Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence.

    Compositions and processes for treatment with lipases
    2.
    发明授权
    Compositions and processes for treatment with lipases 有权

    公开(公告)号：US10731110B2

    公开(公告)日：2020-08-04

    申请号：US15104265

    申请日：2014-12-18

    Applicant: Novozymes A/S

    Inventor： Marc Dominique Morant ,  Anna Verena Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC: C11D3/386 ,  C11D1/00 ,  C11D1/04 ,  C11D1/12 ,  C11D1/40 ,  C11D3/20 ,  C11D3/28 ,  C11D3/36

    Abstract: The present invention relates to a composition comprising: (a) at least one surfactant, at least one surfactant system, at least one soap, or any mixtures thereof; and (b) a polypeptide having lipase activity selected from the group consisting of: (I) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2; (II) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i); (III) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1; (IV) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and (V) a fragment of the polypeptide of (I), (II), (III), or (IV); wherein said composition is a cleaning and/or treatment composition. The invention furthermore relates to methods of producing and using the composition.

    Methods for producing lipases
    6.
    发明授权
    Methods for producing lipases 有权

    公开(公告)号：US10683489B2

    公开(公告)日：2020-06-16

    申请号：US15310247

    申请日：2015-05-26

    Applicant: Novozymes A/S

    Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

    IPC: C12N9/20

    Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence.

    Polypeptides with lipase activity and polynucleotides encoding same
    8.
    发明授权
    Polypeptides with lipase activity and polynucleotides encoding same 有权

    公开(公告)号：US09631164B2

    公开(公告)日：2017-04-25

    申请号：US14770006

    申请日：2014-03-19

    Applicant: Novozymes A/S

    Inventor： Marc Dominique Morant ,  Anna Verana Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC: C12N9/20 ,  C12Q1/34 ,  C12Q1/44 ,  C07H21/04 ,  C11D3/00 ,  C11D7/44 ,  C12S11/00 ,  C11D3/386 ,  C12P13/00

    CPC classification number: C11D3/38627 ,  C11D3/38681 ,  C12N9/20 ,  C12P13/008

    Abstract: The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3 or the full-length complement of (i); (c) a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3; (d) a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and (e) a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    Polypeptides with Lipase Activity And Polynucleotides Encoding Same
    9.
    发明申请
    Polypeptides with Lipase Activity And Polynucleotides Encoding Same 审中-公开
    具有脂肪酶活性的多肽和编码相同的多核苷酸

    公开(公告)号：US20160160196A1

    公开(公告)日：2016-06-09

    申请号：US14902679

    申请日：2014-07-08

    Applicant: NOVOZYMES A/S

    Inventor： Marc Dominique Morant ,  Anna Verena Reiser ,  Carsten Hoerslev Hansen ,  Kim Borch ,  Lone Baunsgaard ,  Vikram Kjoeller Bhatia

    IPC: C12N9/20 ,  C11D3/386

    CPC classification number: C12N9/20 ,  C11D3/38627 ,  C11D3/38681 ,  C12Y301/01 ,  C12Y301/01003 ,  D06L4/40

    Abstract: The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of: (a) a polypeptide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 2; (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 1 or the full-length complement of (i); (c)a polypeptide encoded by a polynucleotide having at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 1; (d) a polypeptide which is a variant of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d). The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

    Abstract translation: 本发明涉及具有脂肪酶活性的分离的多肽，其选自：（a）多肽，其具有至少80％，至少85％，至少90％，至少91％，至少92％，在 与SEQ ID NO：2的至少93％，至少94％，至少95％，至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （b）由多核苷酸编码的多肽，其在低严格条件，中等严格条件，中等严格条件，高严格条件或非常高严格条件下与（i）SEQ ID NO：1或全长补体 的（i）; （c）由多核苷酸编码的多肽，所述多肽具有至少80％，至少85％，至少90％，至少91％，至少92％，至少93％，至少94％，至少95％ 与SEQ ID NO：1的至少96％，至少97％，至少98％，至少99％或100％的序列同一性; （d）在一个或多个（例如多个）位置上包含取代，缺失和/或插入的SEQ ID NO：2的变体的多肽; 和（e）作为（a），（b），（c）或（d）的多肽的片段的多肽。 本发明还涉及编码多肽的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。

    Methods for Producing Lipases
    10.
    发明申请
    Methods for Producing Lipases 审中-公开

    公开(公告)号：US20200255812A1

    公开(公告)日：2020-08-13

    申请号：US16865744

    申请日：2020-05-04

    Applicant: Novozymes A/S

    Inventor： Allan Svendsen ,  Rakhi Saikia ,  Sohel Dalal ,  Vikram Kjoeller Bhatia ,  Lone Baunsgaard

    IPC: C12N9/20

    Abstract: The invention relates to a method for generating a preparation of lipase with increased lipolytic activity comprising a step of altering one or more nucleotides in a polynucleotide comprising a first polynucleotide encoding a propeptide operationally linked to a second polynucleotide encoding a lipase, wherein the alteration of one or more nucleotides are made in the first polynucleotide and the alteration independently results in a substitution, an insertion or a deletion in the encoded propeptide amino acid sequence

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