公开(公告)号：US20180251753A1

公开(公告)日：2018-09-06

申请号：US15972856

申请日：2018-05-07

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Olga PAKHOMOVA ,  Andrei G. PAKHOMOV

IPC: C12N13/00 ,  A61N1/32

CPC classification number: C12N13/00 ,  A61N1/327

Abstract: Methods of enhancing membrane permeabilization in a cell are provided. A method includes disposing the cell between a first electrode and a second electrode and applying a plurality of electrical pulses between the first electrode and the second electrode. In the method, the plurality of electrical pulses include at least two trains of pulses separated by an interval greater than about 10 s. Further, the amplitude of the electrical pulses is selected to be greater than about 0.2 kV/cm.

公开(公告)号：US20250025692A1

公开(公告)日：2025-01-23

申请号：US18713624

申请日：2022-09-23

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Andrei G. PAKHOMOV ,  Vitalii KIM ,  Iurii SEMENOV ,  Emily GUDVANGEN

IPC: A61N1/36 ,  A61N1/04

Abstract: Methods and apparatuses for applying energy to a target tissue or cell are described herein. These methods and apparatuses may use one or more pairs of stimulating electrodes and a dedicated ground electrode to locally apply energy to a target tissue or cell at or adjacent the dedicated ground electrode.

公开(公告)号：US20200147371A1

公开(公告)日：2020-05-14

申请号：US16669419

申请日：2019-10-30

Applicant: Old Dominion University Research Foundation

Inventor： Andrei G. PAKHOMOV ,  Shu XIAO ,  Olga N. PAKHOMOVA ,  Maura CASCIOLA

IPC: A61N1/36 ,  A61N1/04 ,  A61N1/08

Abstract: Methods and apparatuses (systems, devices, etc.) for treating biological tissue to evoke one or more desirable biological and/or physiological effects using pulsed electric fields in the sub-microsecond range at very low electric field strength (e.g., less than 1 kV/cm) but at high (e.g., megahertz) frequencies.

公开(公告)号：US20180050215A1

公开(公告)日：2018-02-22

申请号：US15554808

申请日：2016-03-02

Applicant: OLD DOMINION UNIVERSITY RESEARCH FOUNDATION

Inventor： Andrei G. PAKHOMOV ,  Christian W. ZEMLIN

IPC: A61N1/39

CPC classification number: A61N1/3906 ,  A61N1/327

Abstract: Methods for terminating fibrillation in a fibrillating heart employing nanosecond pulsed electric fields (nsPEFs) are disclosed. nsPEF defibrillation demonstrates its effectiveness as a new defibrillation modality, achieving reliable defibrillation with energies that are an order of magnitude lower than those needed for conventional defibrillation (millisecond shocks with mono- and bi-phasic waveforms). Tests did not reveal any negative effect of nsPEF defibrillation on cardiac tissue, in particular, cardiac tissue treated with nsPEFs does not exhibit a baseline shift in the optical transmembrane potential signal (distinctive feature that indicates electroporation), or changes in action potential duration or shape. The mechanism of nsPEF defibrillation is likely different from conventional defibrillation since it does not rely on membrane charging but on the basis of displacement currents that flow within nanoseconds after the shock is applied. nsPEFs provide the technology for the next generation of defibrillators that help emergency medical services to treat patients effectively.

公开(公告)号：US20210268274A1

公开(公告)日：2021-09-02

申请号：US17306314

申请日：2021-05-03

Applicant: Old Dominion University Research Foundation

Inventor： Andrei G. PAKHOMOV ,  Olga N. PAKHOMOVA ,  Shu XIAO

IPC: A61N1/32 ,  C12N13/00

Abstract: Provided herein are methods of generating a biologically effective unipolar nanosecond electric pulse by superposing two biologically ineffective bipolar nanosecond electric pulses and related aspects, such as electroporation and/or therapeutic applications of these methods to non-invasively target electrostimulation (ES) selectively to deep tissues and organs.

公开(公告)号：US20190054294A1

公开(公告)日：2019-02-21

申请号：US16104089

申请日：2018-08-16

Applicant: Old Dominion University Research Foundation

Inventor： Andrei G. PAKHOMOV ,  Olga N. PAKHOMOVA ,  Shu XIAO

IPC: A61N1/32 ,  A61N1/04 ,  A61N1/36 ,  C12N13/00

Abstract: Provided herein are methods of generating a biologically effective unipolar nanosecond electric pulse by superposing two biologically ineffective bipolar nanosecond electric pulses and related aspects, such as electroporation and/or therapeutic applications of these methods to non-invasively target electrostimulation (ES) selectively to deep tissues and organs.