公开(公告)号：US20120034697A1

公开(公告)日：2012-02-09

申请号：US13206637

申请日：2011-08-10

申请人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garisson ,  William M. Ainley ,  Narasimha C. Samboju

发明人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garisson ,  William M. Ainley ,  Narasimha C. Samboju

IPC分类号： C12N5/04

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12N15/8201

摘要： The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

摘要翻译： 本发明提供简单且一致的方法来将悬浮细胞聚集体破碎成具有完整原始细胞壁的单个细胞。 本发明部分涉及在包含果胶降解酶或包含秋水仙素的微管蛋白去聚合化合物的培养基中培养的悬浮细胞聚集体的细胞分离。 本发明还涉及化合物用于这些目的的新用途。 本发明的另一方面涉及受试者，分离的细胞的转化。 这样的过程简化并将基于单细胞的转化和选择过程整合到转基因和转基因组事件生成工作过程中。 本发明还消除了技术限制，并以高通量方式产生无标记和均匀表达的转基因品系，以支持动物健康，生物制药和性状和作物保护平台的各种需要。