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18 条记录 (耗时 0.021 秒)

    IN VITRO METHODS FOR THE INDUCTION AND MAINTENANCE OF PLANT CELL LINES AS SINGLE SUSPENSION CELLS WITH INTACT CELL WALLS, AND TRANSFORMATION THEREOF
    2.
    发明申请
    IN VITRO METHODS FOR THE INDUCTION AND MAINTENANCE OF PLANT CELL LINES AS SINGLE SUSPENSION CELLS WITH INTACT CELL WALLS, AND TRANSFORMATION THEREOF 有权
    用于诱导和维持植物细胞系作为具有细胞壁的单个悬浮细胞的体外方法及其转化

    公开(公告)号：US20120034697A1

    公开(公告)日：2012-02-09

    申请号：US13206637

    申请日：2011-08-10

    申请人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garisson ,  William M. Ainley ,  Narasimha C. Samboju

    发明人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garisson ,  William M. Ainley ,  Narasimha C. Samboju

    IPC分类号： C12N5/04

    CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12N15/8201

    摘要： The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

    摘要翻译： 本发明提供简单且一致的方法来将悬浮细胞聚集体破碎成具有完整原始细胞壁的单个细胞。 本发明部分涉及在包含果胶降解酶或包含秋水仙素的微管蛋白去聚合化合物的培养基中培养的悬浮细胞聚集体的细胞分离。 本发明还涉及化合物用于这些目的的新用途。 本发明的另一方面涉及受试者，分离的细胞的转化。 这样的过程简化并将基于单细胞的转化和选择过程整合到转基因和转基因组事件生成工作过程中。 本发明还消除了技术限制，并以高通量方式产生无标记和均匀表达的转基因品系，以支持动物健康，生物制药和性状和作物保护平台的各种需要。

    In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls, and transformation thereof
    3.
    发明授权
    In vitro methods for the induction and maintenance of plant cell lines as single suspension cells with intact cell walls, and transformation thereof 有权
    诱导和维持作为具有完整细胞壁的单一悬浮细胞的植物细胞系的体外方法及其转化

    公开(公告)号：US08012752B2

    公开(公告)日：2011-09-06

    申请号：US11965543

    申请日：2007-12-27

    申请人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

    发明人： P. Samuel Jayakumar ,  Jeffrey Beringer ,  Paul R. Schmitzer ,  Frank Burroughs ,  Robbi J. Garrison ,  William M. Ainley ,  Narasimha C. Samboju

    IPC分类号： C12N5/00 ,  C12N5/02

    CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12N15/8201

    摘要： The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

    摘要翻译： 本发明提供简单且一致的方法来将悬浮细胞聚集体破碎成具有完整原始细胞壁的单个细胞。 本发明部分涉及在包含果胶降解酶或包含秋水仙素的微管蛋白去聚合化合物的培养基中培养的悬浮细胞聚集体的细胞分离。 本发明还涉及化合物用于这些目的的新用途。 本发明的另一方面涉及受试者，分离的细胞的转化。 这样的过程简化并将基于单细胞的转化和选择过程整合到转基因和转基因组事件生成工作过程中。 本发明还消除了技术限制，并以高通量方式产生无标记和均匀表达的转基因品系，以支持动物健康，生物制药和性状和作物保护平台的各种需要。

    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    7.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 有权
    用于植物基因工程的土地垫

    公开(公告)号：US20110191899A1

    公开(公告)日：2011-08-04

    申请号：US13011735

    申请日：2011-01-21

    申请人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebbeca R. Miles ,  Steven R. Webb

    发明人： William M. Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Corbin ,  Rebbeca R. Miles ,  Steven R. Webb

    IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

    摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    摘要翻译： 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics
    9.
    发明授权
    Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics 有权
    甘蔗杆菌病毒（SCBV）增强子及其在植物功能基因组学中的应用

    公开(公告)号：US08785612B2

    公开(公告)日：2014-07-22

    申请号：US13220564

    申请日：2011-08-29

    申请人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

    发明人： John P. Davies ,  Vaka S. Reddy ,  William M. Ainley ,  Mark A. Thompson

    IPC分类号： C12N15/00 ,  C12N15/09 ,  C12N15/113 ,  C12N5/04 ,  C12N15/82

    CPC分类号： C12Q1/701 ,  A01H5/10 ,  C12N15/8205 ,  C12N15/8216 ,  C12N15/8261 ,  C12Q2600/13 ,  Y02A40/146

    摘要： Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

    摘要翻译： 鉴定新的增强子序列在植物功能基因组学中具有显着的效用。 已经鉴定出甘蔗杆状坏死病毒（SCBV）转录增强子。 该增强子可用于增加基因启动子的转录速率和激活标记实验。 当将4×阵列的SCBV增强子置于玉米醇脱氢酶最小启动子的截短形式的上游时，观察到转录增加10倍。 描述了使用SCBV转录增强子的方法，嵌合转录调控区，构建体，细胞，组织和包含增强子一个或多个拷贝的生物体也是如此。