公开(公告)号：US20200247831A1

公开(公告)日：2020-08-06

申请号：US16642439

申请日：2018-08-31

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Hye Gun RYU ,  Kyeong Hwan KIM ,  Yong Woong JUN ,  Subhankar SINGHA

IPC: C07F7/08 ,  A61K49/00

Abstract: The present invention relates to a silicon-substituted amino-pyronin compound derivative, a cell- or tissue-imaging method using the same, a cancer diagnosis method using the same, and a method for preparation of the same. More specifically, serving as a two-photon absorption fluorescent substance which has longer absorption and emission wavelengths in red or near-infrared regions of 625 nm or greater, compared to conventional one-photon absorption fluorescent substances, the silicon-substituted amino-pyronin compound derivative can minimize the influence of self-fluorescence in a bioimaging study and is thus expected to be suitable for high-resolution imaging in deep tissues. A fluorescent probe developed on the basis of the fluorescent substance platform is expected to find an expanded range of applications in analyzing and imaging specific materials in vivo.

公开(公告)号：US20160377605A1

公开(公告)日：2016-12-29

申请号：US15194188

申请日：2016-06-27

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Yong Woong JUN ,  Donghee MA

IPC: G01N33/53 ,  G01N33/58 ,  G01N21/64 ,  C07F5/02

CPC classification number: G01N33/5308 ,  C07F5/022 ,  C12Q1/008 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5735 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2400/00

Abstract: A fluorescent probe compound, a preparation method thereof, and a method of imaging and quantifying lysosomal adenosine triphosphate (ATP) at a cell or tissue level through one-photon or two-photon fluorescence microscopy using the compound is disclosed. A fluorescence detection system capable of detecting lysosomal ATP is further disclosed. Since the fluorescent probe compound is found to be capable of selectively sensing and quantifying lysosomal ATP in a cell or tissue, it is expected that the disclosed compound or composition can be usefully employed in the study of various biological reactions or diseases associated with ATP in a living body.

Abstract translation: 公开了一种荧光探针化合物及其制备方法，以及通过使用该化合物的单光子或双光子荧光显微镜在细胞或组织水平上成像和定量溶酶体三磷酸腺苷（ATP）的方法。 进一步公开了能够检测溶酶体ATP的荧光检测系统。 由于发现荧光探针化合物能够选择性地感测和定量细胞或组织中的溶酶体ATP，所以预期所公开的化合物或组合物可有效地用于研究各种生物反应或与ATP相关的疾病 活体。