公开(公告)号：US20250163354A1

公开(公告)日：2025-05-22

申请号：US18837988

申请日：2022-07-21

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Dong Sung KIM ,  Hyeon Beom CHONG ,  Jun Yeol RHYOU ,  Jae Seung YOUN

IPC: C12M1/32 ,  C12M1/12 ,  C12M1/42 ,  C12M3/00

Abstract: The present invention may provide a cell culture device and a cell culture system comprising same, wherein the cell culture device comprises: a plurality of upper chambers provided with a porous membrane on the lower side thereof and first accommodation spaces thereinside; a lower chamber configured to provide a second accommodation spaces therein; and a support configured to prevent the plurality of upper chambers from coming into contact with the lower chamber and configured such that the plurality of upper chambers are spaced apart from each other in a horizontal direction by a predetermined distance, wherein the first accommodation spaces and the second accommodation spaces are configured to exchange materials with each other through the porous membrane, and the second accommodation spaces have a material exchange area common to each of the first accommodation spaces provided in the plurality of upper chambers.

公开(公告)号：US20210340475A1

公开(公告)日：2021-11-04

申请号：US17238332

申请日：2021-04-23

Applicant: POSTECH Research and Business Development Foundation ,  Board of Regents, The University of Texas System

Inventor： Dong Sung KIM ,  Woojung Shin ,  Hyun Jung Kim ,  Jae Seung Yoon ,  Hyeon Beom Chong

IPC: C12M1/32 ,  C12M1/04 ,  C12M1/12 ,  C12M1/00 ,  C12M1/34

Abstract: The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.

公开(公告)号：US20240384237A1

公开(公告)日：2024-11-21

申请号：US18641674

申请日：2024-04-22

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Dong Sung KIM ,  Jae Seung YOUN

IPC: C12N5/071 ,  C08J3/075

Abstract: The present disclosure relates to a double-layer structure including: a first layer composed of a gel composition; a second layer formed on the first layer and having an epithelial cell layer as at least a region coming into contact with the first layer; and a wrinkle structure formed thereon by means of compressive forces applied from the outside thereto, the wrinkle structure having at least one or more crests or troughs formed thereon in a state where the first layer and the second layer are connected to each other.