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    Method for Quantitative Evaluation of a Rearrangement or a Targeted Genetic Recombination of an Individual and Uses Thereof
    1.
    发明申请
    Method for Quantitative Evaluation of a Rearrangement or a Targeted Genetic Recombination of an Individual and Uses Thereof 审中-公开
    定量评估个体的重排或靶向遗传重组的方法及其用途

    公开(公告)号：US20080166704A1

    公开(公告)日：2008-07-10

    申请号：US10581814

    申请日：2004-12-03

    申请人： Patrice Marche ,  Evelyne Jouvin-Marche ,  Nicolas Pasqual

    发明人： Patrice Marche ,  Evelyne Jouvin-Marche ,  Nicolas Pasqual

    IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C07H21/04

    CPC分类号： C12Q1/6881 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/16

    摘要： A method for quantitative evaluation of a rearrangement or targeted genetic recombination of an individual and the immunity repertoire of an individual and the uses thereof, particularly in the case of follow-up to a treatment or in the diagnosis and/or prognosis of certain pathologies. Said method comprises at least: (a) extraction of human genomic DNA from a biological sample, (b) amplification of a segment of said genomic DNA, the size thereof ranging from a few hundreds of base pairs and several quantities of ten kb by multiplex PCR in the presence of: one or several couples of primers, and polymerase DNA or a polymerase DNA mixture enabling the amplification of segments of genomic DNA, the size thereof ranging from several hundreds of base pairs and several quantities of ten kb, having a corrective effect enabling elongation to be substantially improved, said amplification also comprising a stage of initial denaturation, cycles of denaturation, hybridation and elongation and wherein the elongation stages are carried out at least during a period of 10 minutes at 68° C.-72° C.; (c) separation of the amplified DNAg fragments and (d) detection of the rearranged or recombined segments.

    摘要翻译： 一种用于定量评估个体的重排或靶向基因重组的方法以及个体的免疫特性及其用途，特别是在治疗后续或某些病理学的诊断和/或预后的情况下。 所述方法至少包括：（a）从生物样品中提取人基因组DNA，（b）扩增所述基因组DNA的片段，其大小范围从几百个碱基对，若干数量的10kb通过多重 PCR存在下：一对或几对引物，以及聚合酶DNA或聚合酶DNA混合物，其能够扩增基因组DNA区段，其尺寸范围从几百个碱基对和数量的10kb，具有校正 所述扩增还包括初始变性阶段，变性循环，杂交和延伸，并且其中伸长阶段至少在68℃-72℃的10分钟期间内进行 。 （c）扩增的DNAg片段的分离和（d）重排或重组片段的检测。

    Method of Determining the Diversity of T Lymphocytes in a Biological Sample
    2.
    发明申请
    Method of Determining the Diversity of T Lymphocytes in a Biological Sample 审中-公开
    确定生物样品中T淋巴细胞多样性的方法

    公开(公告)号：US20080286777A1

    公开(公告)日：2008-11-20

    申请号：US11883633

    申请日：2006-02-03

    申请人： Serge Candeias ,  Cedric Touvrey ,  Evelyne Jouvin-Marche ,  Patrice Marche

    发明人： Serge Candeias ,  Cedric Touvrey ,  Evelyne Jouvin-Marche ,  Patrice Marche

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q1/6881 ,  C12Q2600/156

    摘要： The invention relates to the field comprising the diagnosis of possible immune system disorders and the analysis of immune responses. In particular, the invention relates to a method of determining the diversity of T lymphocytes in a biological sample, based on the molecular analysis of the structure of the junctions resulting from the recombination rearrangement V(D)J of element δRec-1 with an AJ gene. More specifically, the invention relates to a method of analyzing the combinatorial diversity and/or the junctional diversity of the excision circles (TREC) resulting from the rearrangement of δRec-1, in order to determine the heterogeneity of a given population of T lymphocytes.

    摘要翻译： 本发明涉及包括可能的免疫系统疾病的诊断和免疫应答分析的领域。 特别地，本发明涉及一种基于由元件deltaRec-1与AJ的重组重排V（D）J产生的连接结构的分子分析来确定生物样品中T淋巴细胞多样性的方法 基因。 更具体地，本发明涉及分析由ΔRec-1重排产生的切除圆（TREC）的组合多样性和/或连接多样性的方法，以便确定给定群体的T淋巴细胞的异质性。