公开(公告)号：US20250073765A1

公开(公告)日：2025-03-06

申请号：US18949739

申请日：2024-11-15

Applicant: QINGDAO UNIVERSITY OF TECHNOLOGY

Inventor： Huawei WANG ,  Xiqing CUI ,  Zhibo YANG ,  Yanan WANG ,  Xueqin WANG ,  Rongxue ZOU ,  Xiangyu MENG ,  Yuewei YANG ,  Yujun WANG ,  Yingjie SUN ,  Yuanwen LIU ,  Haoran DING

IPC: B09C1/10 ,  B09C1/08 ,  C12N1/20 ,  C12R1/38

Abstract: A microbial preparation and a method for remedying a polycyclic aromatic hydrocarbons (PAHs)-contaminated soil are disclosed. The microbial preparation contains Pseudomonas sp. KW-2 with a deposit number of CGMCC No. 29017. In the soil remediation method, an oxidizing agent is added to the PAHs-contaminated soil for pre-oxidation treatment, where the oxidizing agent is selected from the group consisting of Fenton's reagent, hydrogen peroxide, sodium persulfate, potassium permanganate, and a combination thereof; a carbon source or a nitrogen source added to reach a carbon-to-nitrogen ratio of 100:5-20, and the microbial preparation is introduced for microbial remediation, where the carbon source is selected from the group consisting of glucose, fructose, sucrose, starch, and a combination thereof; and the nitrogen source is selected from the group consisting of ammonium chloride, ammonium nitrate, potassium nitrate, sodium nitrate, and a combination thereof.

公开(公告)号：US20240060036A1

公开(公告)日：2024-02-22

申请号：US18145911

申请日：2022-12-23

Applicant: Qingdao University of Technology

Inventor： Huawei WANG ,  Xutong JIANG ,  Yanru ZHANG ,  Jing SONG ,  Yanan WANG ,  Yingjie SUN ,  Shupeng LI ,  Lewei YANG ,  Yuanwen LIU

IPC: C12N1/20 ,  B09C1/10

CPC classification number: C12N1/205 ,  B09C1/10 ,  C12R2001/38

Abstract: Pseudomonas sp. strain KW-2.

公开(公告)号：US20200164417A1

公开(公告)日：2020-05-28

申请号：US16737871

申请日：2020-01-08

Applicant: Qingdao University of Technology

Inventor： Huawei WANG ,  Zijuan LV ,  Yanan WANG ,  Yingjie SUN

IPC: B09C1/10 ,  C12P3/00 ,  C12N1/20

Abstract: A method of in-situ remediation of arsenic-contaminated soil, the method including: 1) inoculating a bacterial strain including Pseudomonas putida MnB1 in a culture medium where the addition amount of the bacterial strain accounts for 2-10% (v/v) of the culture medium; shaking the culture medium including the bacterial strain at the rotation speed of 100-180 rpm at 15-35° C. for 1-5 days under the aerobic condition thereby yielding an enriched bacterial strain; and 2) adding the enriched bacterial strain, manganese carbonate, ammonium ferrous sulfate, sodium citrate, and yeast extract to an arsenic contaminated soil; adding water to the soil until the soil has a moisture content of 50-70%, stirring the soil for 5-30 min, and culturing the bacterial strain in the soil under the aerobic/microaerobic condition at 10-40° C. for 2-6 weeks.

公开(公告)号：US20250042793A1

公开(公告)日：2025-02-06

申请号：US18920837

申请日：2024-10-18

Applicant: QINGDAO UNIVERSITY OF TECHNOLOGY

Inventor： Huawei WANG ,  Rongxue ZOU ,  Zhibo YANG ,  Yanan WANG ,  Xueqin WANG ,  Yingjie SUN ,  Yujun WANG ,  Shupeng LI

IPC: C02F3/34 ,  C01G45/00 ,  C02F3/10 ,  C02F101/10 ,  C12N1/20

Abstract: A manganese carbonate-supported ferrihydrite material preparation method, in which manganese sulfate and ammonium bicarbonate are respectively dissolved to obtain a manganese sulfate solution and an ammonium bicarbonate solution, and the manganese sulfate solution is added with ferrihydrite, dropwise added with the ammonium bicarbonate solution and reacted under magnetic stirring to obtain a precipitate. The precipitate is washed with distilled water and dried in a drying oven to obtain the manganese carbonate-supported ferrihydrite material. A method for treating wastewater polluted by arsenic (As) and antibiotics by using the manganese carbonate-supported ferrihydrite material is also provided.

公开(公告)号：US20250042787A1

公开(公告)日：2025-02-06

申请号：US18920838

申请日：2024-10-18

Applicant: QINGDAO UNIVERSITY OF TECHNOLOGY

Inventor： Huawei WANG ,  Xueqin WANG ,  Rongxue ZOU ,  Xiqing CUI ,  Mengfan LIU ,  Yanan WANG ,  Zhibo YANG ,  Shupeng LI ,  Yingjie SUN ,  Yujun WANG

IPC: C02F1/72 ,  C02F3/34 ,  C02F101/34 ,  C12N1/20

Abstract: A method for preparing a biogenic manganese oxide (BMO)@sponge biomaterial, in which a divalent manganese ion solution and a sponge carrier are added to a pre-sterilized culture medium, and then a manganese oxidizing bacterium with an accession number of ATCC 23483 is inoculated. The culture medium was cultured and freeze-dried to obtain the BMO@sponge biomaterial. This application further provides a method for treating an antibiotic-containing wastewater, in which a BMO@sponge biomaterial prepared by such method and a peroxymonosulfate (PMS) added to the wastewater. The PMS is activated by the BMO@sponge biomaterial to generate active substances such as hydroxyl radicals, sulfate radicals and singlet oxygen radicals, which can efficiently degrade the antibiotic into CO2 and water through a series of physical and chemical reactions and free radical chain reactions.

公开(公告)号：US20240059593A1

公开(公告)日：2024-02-22

申请号：US18499139

申请日：2023-10-31

Applicant: Qingdao University of Technology

Inventor： Huawei WANG ,  Yanan WANG ,  Yingjie SUN ,  Jing SONG ,  Mengfan LIU ,  Yanru ZHANG ,  Xutong JIANG

IPC: C02F1/72 ,  C12N1/20 ,  C12P3/00 ,  C02F1/66 ,  B01J23/00 ,  B01J37/36 ,  B01J37/32

CPC classification number: C02F1/725 ,  C12N1/205 ,  C12P3/00 ,  C02F1/66 ,  B01J23/002 ,  B01J37/36 ,  B01J37/32 ,  C12R2001/38

Abstract: A method of preparing a FeMnCeOx biomaterial is provided, including the following steps. A Pseudomonas sp. strain KW-2 is obtained. A culture medium with a pH of 6.5-7.8 is prepared, which includes 0.1 g/L K2HPO4, 0.2 g/L MnSO4·7H2O, 0.2 g/L NaNO3, 0.1 g/L CaCl2), 0.1 g/L NH4Cl, 0.1 g/L (NH4)2CO3, 35 g/L NaCl and 150 mg/L ferric ammonium citrate. The culture medium is autoclaved, inoculated with the KW-2 strain, cultured for 1-3 days, added with a cerium nitrate solution, cultured for 3-7 days and centrifuged at 4,000-8,000 rpm for 10-20 min to collect a precipitate. The precipitate is rinsed 5-8 times with deionized water and 0.01 mol/L phosphate buffered saline (PBS) and freeze-dried at −60° C. to obtain the FeMnCeOx biomaterial. A method for treating antibiotic wastewater using the FeMnCeOx biomaterial is also provided.