公开(公告)号：US20040166505A1

公开(公告)日：2004-08-26

申请号：US10631573

申请日：2003-07-30

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  R. Hugh Daniels ,  Stephen A. Empedocles ,  Vince E. Phillips ,  Edith Y. Wong ,  Donald A. Zehnder

IPC: C12Q001/68

CPC classification number: G01N33/54346 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/588 ,  G01N2458/00 ,  Y10S977/924

Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.

Abstract translation: 公开了在各种化学和生物应用中使用半导体纳米晶体作为可检测标记。 该方法用于通过使用多于一种半导体纳米晶体作为可检测标记来检测单个分析物以及多个分析物，每个分析物以不同的波长发射。

公开(公告)号：US20010034034A1

公开(公告)日：2001-10-25

申请号：US09887914

申请日：2001-06-21

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez ,  R. Hugh Daniels ,  Stephen A. Empedocles ,  Vince E. Phillips ,  Edith Y. Wong ,  Donald A. Zehnder

IPC: C12Q001/68

CPC classification number: G01N33/54346 ,  B82Y15/00 ,  G01N33/533 ,  G01N33/588 ,  G01N2458/00 ,  Y10S977/924

Abstract: The use of semiconductor nanocrystals as detectable labels in various chemical and biological applications is disclosed. The methods find use for detecting a single analyte, as well as multiple analytes by using more than one semiconductor nanocrystal as a detectable label, each of which emits at a distinct wavelength.

公开(公告)号：US20030165951A1

公开(公告)日：2003-09-04

申请号：US10331285

申请日：2002-12-30

Applicant: Quantum Dot Corporation

Inventor： Marcel P. Bruchez JR. ,  Jennifer H. Lai ,  Vince E. Phillips ,  Andrew R. Watson ,  Edith Y. Wong

IPC: C12Q001/68 ,  C12M001/34

CPC classification number: B82Y5/00 ,  B82Y10/00 ,  C12Q1/6818 ,  C12Q1/6834 ,  C12Q2537/143 ,  C12Q2561/119 ,  C12Q2525/301

Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide and/or an amplification product therefrom are provided. The methods comprise contacting a sample suspected of containing the target polynucleotide with a polynucleotide that can bind specifically thereto; this polynucleotide is conjugated to a substrate, preferably an encoded bead conjugate. An amplification reaction can first be used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay for the target polynucleotide. An amplification product detection complex and method of forming the same are also provided. The methods are particularly useful in multiplex settings where a plurality of targets are present. Amplification product assay complexes and amplification product assay arrays are also provided, along with methods of forming the same. Kits comprising reagents for performing such methods are also provided.

公开(公告)号：US20020009728A1

公开(公告)日：2002-01-24

申请号：US09766273

申请日：2001-01-18

Applicant: Quantum Dot Corporation

Inventor： Michael Bittner ,  Edith Y. Wong ,  Marcel P. Bruchez JR.

IPC: C12Q001/68

CPC classification number: C12Q1/6834 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q2600/158 ,  C12Q2563/155

Abstract: Methods for assaying a sample for a probe polynucleotide are provided. The methods comprise forming a complex between a target on a substrate, the probe polynucleotide that binds to the target, and a conjugate comprising a semiconductor nanocrystal that binds to the probe polynucleotide by way of a tag sequence on the probe polynucleotide. The complex is formed when the probe polynucleotide is present in the sample. The methods are useful in any technique in which the detection of a target that can bind to a probe polynucleotide is desired, for example in fluorescence in situ hybridization. The methods are particularly useful in multiplex settings such as hybridization to microarrays where a plurality of targets are present. Assay complexes produced by such methods and kits useful for performing such methods are also provided.

Abstract translation: 提供了用于测定探针多核苷酸样品的方法。 所述方法包括在底物上的靶，与靶标结合的探针多核苷酸和包含通过探针多核苷酸上的标签序列结合探针多核苷酸的半导体纳米晶体的缀合物之间形成复合物。 当探针多核苷酸存在于样品中时形成复合物。 所述方法可用于其中期望检测可结合探针多核苷酸的靶的任何技术，例如在荧光原位杂交中。 所述方法在多重设置中是特别有用的，例如与存在多个靶的微阵列的杂交。 还提供了通过用于实施这些方法的这些方法和试剂盒产生的测定复合物。