公开(公告)号：US20140308688A1

公开(公告)日：2014-10-16

申请号：US14362942

申请日：2012-12-07

Applicant: Research Triangle Institute

Inventor： Sonia Grego ,  Brian Rhys Stoner ,  Kristin Hedgepath Gilchrist ,  Timothy Raymond Fennell ,  Ann Pitruzzello

IPC: G01N33/50

CPC classification number: G01N33/5014 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/028 ,  B01L2300/0645 ,  B01L2400/0481 ,  B01L2400/0487 ,  C12M21/08 ,  C12M23/16 ,  C12M23/44 ,  C12M35/08 ,  G01N33/5082

Abstract: A multiple flow-based microfluidic cell culture system that emulates mammalian physiology is provided. Tissue-mimicking cell cultures are connected by flow within a physiologically meaningful arrangement so that the pharmacokinetics of various agents to be tested in the system emulate in vivo conditions. The system includes at least two organ tissue modules, each organ tissue module including a first chamber containing an organ tissue cell, the first chamber including an inlet and an outlet for flow of an organ tissue cell-specific culture medium; a second chamber including an inlet and an outlet for flow of a blood material; and a semi-permeable membrane separating the first and second chambers. The flow of blood material through each organ tissue module is interconnected and the flow of tissue-cell specific culture medium is directed to a single organ tissue module.

Abstract translation: 提供了一种模拟哺乳动物生理学的基于多流的微流体细胞培养系统。 组织模拟细胞培养物在生理上有意义的排列下通过流动连接，使得在系统中待测试的各种试剂的药代动力学模拟体内条件。 所述系统包括至少两个器官组织模块，每个器官组织模块包括含有器官组织细胞的第一腔室，所述第一腔室包括用于器官组织细胞特异性培养基流动的入口和出口; 第二室，包括用于血液材料流动的入口和出口; 以及分离第一和第二室的半透膜。 通过每个器官组织模块的血液材料的流动相互连接，并且组织细胞特异性培养基的流动被引导到单个器官组织模块。