公开(公告)号：US20140308688A1

公开(公告)日：2014-10-16

申请号：US14362942

申请日：2012-12-07

Applicant: Research Triangle Institute

Inventor： Sonia Grego ,  Brian Rhys Stoner ,  Kristin Hedgepath Gilchrist ,  Timothy Raymond Fennell ,  Ann Pitruzzello

IPC: G01N33/50

CPC classification number: G01N33/5014 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2200/028 ,  B01L2300/0645 ,  B01L2400/0481 ,  B01L2400/0487 ,  C12M21/08 ,  C12M23/16 ,  C12M23/44 ,  C12M35/08 ,  G01N33/5082

Abstract: A multiple flow-based microfluidic cell culture system that emulates mammalian physiology is provided. Tissue-mimicking cell cultures are connected by flow within a physiologically meaningful arrangement so that the pharmacokinetics of various agents to be tested in the system emulate in vivo conditions. The system includes at least two organ tissue modules, each organ tissue module including a first chamber containing an organ tissue cell, the first chamber including an inlet and an outlet for flow of an organ tissue cell-specific culture medium; a second chamber including an inlet and an outlet for flow of a blood material; and a semi-permeable membrane separating the first and second chambers. The flow of blood material through each organ tissue module is interconnected and the flow of tissue-cell specific culture medium is directed to a single organ tissue module.

Abstract translation: 提供了一种模拟哺乳动物生理学的基于多流的微流体细胞培养系统。 组织模拟细胞培养物在生理上有意义的排列下通过流动连接，使得在系统中待测试的各种试剂的药代动力学模拟体内条件。 所述系统包括至少两个器官组织模块，每个器官组织模块包括含有器官组织细胞的第一腔室，所述第一腔室包括用于器官组织细胞特异性培养基流动的入口和出口; 第二室，包括用于血液材料流动的入口和出口; 以及分离第一和第二室的半透膜。 通过每个器官组织模块的血液材料的流动相互连接，并且组织细胞特异性培养基的流动被引导到单个器官组织模块。

公开(公告)号：US20140335496A1

公开(公告)日：2014-11-13

申请号：US14362419

申请日：2012-12-04

Applicant: Research Triangle Institute ,  The University of North Carolina at Chapel Hill

Inventor： Sonia Grego ,  Kristin Hedgepath Gilchrist ,  Scott H. Randell

IPC: G09B23/30

CPC classification number: G09B23/306 ,  C12M21/08 ,  C12M23/16 ,  C12M25/02 ,  C12M25/14 ,  C12M35/08 ,  G01N33/5088

Abstract: A multicellular fluidic enhanced airway model system of the conducting airways as a tool for the evaluation of biological threats and medical countermeasures is provided. The airway model system can include a first chamber having an inlet and an outlet and containing epithelial cells; a second chamber having an inlet and an outlet and containing an extracellular matrix, wherein the second chamber is separated from the first chamber by a porous membrane; and a third chamber having an inlet and an outlet, wherein the third chamber is separated from the second chamber by a porous membrane, and wherein the airway tissue model system is configured to provide a separate fluidic pathway through each of said first, second, and third chambers. A method of analyzing tissue response to an agent via an airway tissue model system is also provided.

Abstract translation: 提供了导航气道的多细胞流体强化气道模型系统作为评估生物威胁和医疗对策的工具。 气道模型系统可以包括具有入口和出口并包含上皮细胞的第一腔室; 具有入口和出口并且包含细胞外基质的第二腔室，其中所述第二腔室通过多孔膜与所述第一腔室分离; 以及具有入口和出口的第三腔室，其中所述第三腔室通过多孔膜与所述第二腔室分离，并且其中所述气道组织模型系统构造成提供通过所述第一，第二和第 第三室。 还提供了一种通过气道组织模型系统分析对试剂的组织反应的方法。

公开(公告)号：US20210251558A1

公开(公告)日：2021-08-19

申请号：US17236266

申请日：2021-04-21

Applicant: Research Triangle Institute

Inventor： Kristin Hedgepath Gilchrist ,  Sonia Grego ,  Gregory Frederick Lewis ,  Meghan Sarah Hegarty-Craver

IPC: A61B5/00 ,  A61B5/352 ,  A61B5/11 ,  A61B5/0205 ,  G16H50/30

Abstract: A computer implemented method is disclosed for detecting a mild traumatic brain injury (mTBI) on a subject having recently suffered a head injury. The method includes (1) receiving first electrocardiogram (ECG) data from the subject during a first transition from a first position to a second position; and (2) determining a probability value of mTBI within the subject based at least partially on the first ECG data during the first transition. The first position may be a prone position or a supine position and the second position may be a standing position.