公开(公告)号：US20210079422A1

公开(公告)日：2021-03-18

申请号：US16627227

申请日：2018-06-29

Applicant: Spark Therapeutics, Inc.

Inventor： Younghoon OH ,  Guang QU

IPC: C12N15/86 ,  B01D15/34 ,  B01D15/36

Abstract: Described and provided herein are purification, production and manufacturing methods for recombinant adeno-associated viral (rAAV) vector particles. Purification, production and manufacturing methods set forth herein, for example, include at least 2 column chromatography steps. Column chromatography steps include, for example, cation exchange chromatography, anion exchange chromatography, size exclusion chromatography and/or AAV affinity chromatography alone or in combination and in any order.

公开(公告)号：US20200299650A1

公开(公告)日：2020-09-24

申请号：US16088743

申请日：2017-03-31

Applicant: SPARK THERAPEUTICS, INC.

Inventor： Guang QU ,  Younghoon OH ,  Lin LU ,  John Fraser WRIGHT

IPC: C12N7/00 ,  C12N1/06 ,  B01D15/36 ,  B01D15/34 ,  B01D61/14 ,  B01D15/38

Abstract: In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.

公开(公告)号：US20190284576A1

公开(公告)日：2019-09-19

申请号：US16319216

申请日：2017-07-21

Applicant: Spark Therapeutics, Inc.

Inventor： Guang QU ,  John Fraser WRIGHT ,  Younghoon OH ,  Yuhuan WANG ,  Haibo ZHANG ,  Laura DUNCAN

IPC: C12N15/86 ,  C07K14/755 ,  C12N9/64 ,  C12N9/48

Abstract: Provided are methods for producing recombinant adeno-associated virus (rAAV) vector particles at high recovery or high titer. Also provided are methods that concentrate rAAV vectors to a high concentration, for example, up to 5E+13 (5×1013) vector genomes per milliliter (Vg/ml) with little if any rAAV aggregates.