公开(公告)号：US20170247754A1

公开(公告)日：2017-08-31

申请号：US15443138

申请日：2017-02-27

Applicant: STC.UNM

Inventor： Jeremy Edwards ,  Payman Zarkesh-Ha ,  Steven R.J. Brueck

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  Y10T436/143333 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.

公开(公告)号：US20140234981A1

公开(公告)日：2014-08-21

申请号：US14347713

申请日：2012-09-28

Applicant: STC.UNM

Inventor： Payman Zarkesh-Ha ,  Steven R.J. Brueck ,  Jeremy Edwards

IPC: G01N27/414

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  Y10T436/143333 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: Devices that include a substrate; a source region and a drain region formed within the substrate and having a channel region provided therebetween; a first insulating layer formed over the channel region; a first floating gate formed over the first insulating layer, the first floating gate configured to respond to an analyte in a target material; and a second gate formed over the first floating gate, the second gate capacatively coupled but not electrically connected to the first floating gate.

Abstract translation: 包括基板的装置; 源极区域和漏极区域，形成在所述衬底内并且在其间设置有沟道区域; 形成在所述沟道区上的第一绝缘层; 形成在所述第一绝缘层上的第一浮动栅极，所述第一浮置栅极被配置为响应目标材料中的分析物; 以及形成在所述第一浮动栅极上的第二栅极，所述第二栅极电容耦合但不电连接到所述第一浮动栅极。

公开(公告)号：US09982296B2

公开(公告)日：2018-05-29

申请号：US14870513

申请日：2015-09-30

Applicant: STC.UNM

Inventor： Jeremy Edwards

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2525/155 ,  C12Q2521/313 ,  C12Q2565/518 ,  C12Q2563/131 ,  C12Q2525/191

Abstract: We describe ultra-high throughput polony genome sequencing that can permit, for example, generating raw data to re-sequencing the human genome in about one week (including library prep and sequencing) at a reasonable cost. The methods described herein include one or more of the following: (1) increasing polony sequencing read length, (2) improving library construction and emulsions protocols, (3) increasing bead density and/or moving to alternative clonal amplication strategies (other than emulsion PCR or ePCR), (4) extending software capabilities to allow SNP calls from our new sequencing raw data, (5) Dual Primer Emulsion PCR, and (6) diagnostic method exploiting one or more of the foregoing.

公开(公告)号：US20140329712A1

公开(公告)日：2014-11-06

申请号：US14347690

申请日：2012-09-28

Applicant: STC.UNM

Inventor： Jeremy Edwards ,  Payman Zarkesh-Ha ,  Steven R.J. Brueck

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  Y10T436/143333 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.

Abstract translation: 本公开一方面描述了用于测序的DNA分子的制备方法。 通常，该方法包括将DNA分子片段化成双链片段; 扩增至少一部分双链片段; 使片段环化，使得片段的第一端包含连接线束的第一环和片段的第二端包括连接线的第二环; 将第一测序引物退火至所述第一环，其定向为序列所述片段的一条链的至少一部分; 以及将第二测序引物退火至所述第二环，其定向为序列所述片段的另一条链的至少一部分。 在另一方面，本公开描述了用于测序DNA分子的方法。 通常，该方法包括将DNA分子片段化成双链片段; 扩增至少一部分双链片段; 使片段环化，使得片段的第一端包含连接线束的第一环和片段的第二端包括连接线的第二环; 并测序至少一个DNA链。

公开(公告)号：US20190024164A1

公开(公告)日：2019-01-24

申请号：US16069444

申请日：2017-01-11

Applicant: Paul SZAUTER ,  STC.UNM

Inventor： Payman Zarkesh-Ha ,  Jeremy Edwards ,  Paul Szauter

IPC: C12Q1/6874 ,  G01N27/414

Abstract: This disclosure describes, in one aspect, a method of electric-field-assisted nucleotide sequencing. Generally, the method includes performing an ion-sensitive nucleotide sequencing method, applying an electric field across the device while the nucleotide sequencing reactions are being performed so that ions released by the sequencing reactions are directed to contact with the ion-sensitive detector, and detecting at least a portion of the released ions in contact with the ion-sensitive detector.

公开(公告)号：US09617590B2

公开(公告)日：2017-04-11

申请号：US14347690

申请日：2012-09-28

Applicant: STC.UNM

Inventor： Jeremy Edwards ,  Payman Zarkesh-Ha ,  Steven R. J. Brueck

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  Y10T436/143333 ,  C12Q2531/125 ,  C12Q2535/122

Abstract: This disclosure describes, in one aspect, a method for preparing DNA molecule for sequencing. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the fragment. In another aspect, this disclosure describes a method for sequencing a DNA molecule. Generally, the method includes fragmenting the DNA molecule into double-stranded fragments; amplifying at least a portion of the double-stranded fragments; circularizing the fragments so that the first end of the fragment comprises a first loop connecting the strands and the second end of the fragment comprises a second loop connecting the strands; and sequencing at least one of the DNA strands.