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公开(公告)号:US20180274030A1
公开(公告)日:2018-09-27
申请号:US15779136
申请日:2016-11-25
发明人: Éva PÁLLINGER , Júlia SZEKERES , Zoltán BOGNÁR , Krisztina GÖDÖNY , József BÓDIS , Edit BUZÁS
IPC分类号: C12Q1/6881 , G01N15/14
CPC分类号: C12Q1/6881 , C12Q1/6841 , C12Q1/6883 , G01N15/14 , G01N2015/1486 , C12Q2563/161 , C12Q2565/518
摘要: The invention relates to a non-invasive, simple, inexpensive, quick method to assess embryo competence in vitro by detecting nucleic acid-containing extracellular vesicles in the culture medium of the embryo. The culture medium may be fixed before being stained with propidium iodide and analysed by flow cytometry.
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公开(公告)号:US10059993B2
公开(公告)日:2018-08-28
申请号:US14837931
申请日:2015-08-27
发明人: Ellen H. Fiss , Nicolas Newton
IPC分类号: C12Q1/68 , C12Q1/6876 , C12Q1/6851
CPC分类号: C12Q1/6876 , C12Q1/6851 , C12Q2600/166 , C12Q2521/107 , C12Q2545/101 , C12Q2565/518
摘要: Methods and oligonucleotides are provided for detecting an internal control nucleic acid for qualitative and/or quantitative purposes.
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公开(公告)号:US20180230523A1
公开(公告)日:2018-08-16
申请号:US15882380
申请日:2018-01-29
申请人: Duke University
发明人: Tuan Vo-Dinh , Naveen Gandra , Hoan T. Ngo
IPC分类号: C12Q1/6825 , A61B5/00
CPC分类号: C12Q1/6825 , A61B5/0075 , B82Y15/00 , B82Y20/00 , B82Y30/00 , C12Q2565/518 , C12Q2565/549 , C12Q2565/632 , G01N21/658
摘要: Gold nanorattle probes are provided that are highly tunable, physiologically stable, and ultra-bright Raman probes for in vitro and in vivo surface-enhanced Raman scattering (SERS) applications. The nanorattles contain an essentially uniform gap between core and shell that is tunable and can range from 2nm to 10nm in width. This provides numerous advantages including allowing for increased loading with a variety of dye molecules that exhibit SERS in various spectral regions, including the “tissue optical window” for in vivo studies. In addition, the nanorattle probes provide an internal label when used in diagnostic methods to detect nucleic acids, proteins and other biotargets. The nanorattles have an essentially spherical gold metal nanoparticle core, a porous material of silver metal of an essentially uniform width surrounding the nanoparticle core that is loaded with one or more SERS reporter molecules, and an outer gold metal shell encapsulating the porous material.
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公开(公告)号:US10036063B2
公开(公告)日:2018-07-31
申请号:US13384302
申请日:2010-06-30
申请人: John Stephen West
发明人: John Stephen West
IPC分类号: C12Q1/68 , C07H21/02 , C07H21/04 , C12Q1/6869
CPC分类号: C12Q1/6869 , C12Q2565/518 , C12Q2525/191 , C12Q2525/186
摘要: A method of determining the sequence of a target nucleic acid is provided. The method can include the steps of (a) performing a defined number of incremental extension cycles to produce a population of nucleic acid fragments having different portions of the target nucleic acid wherein the individual nucleic acid fragments in the population have a defined length that is correlated with the number of incremental extension cycles; (b) determining the sequence of the first end of individual nucleic acid fragments in the population, thereby providing first end sequences; (c) determining the sequence of the second end of individual nucleic acid fragments in the population, thereby providing second end sequences; and (d) determining the sequence of the target nucleic acid based on the first end sequences, the second end sequences and the defined length.
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公开(公告)号:US20180155709A1
公开(公告)日:2018-06-07
申请号:US15575803
申请日:2016-05-27
IPC分类号: C12N15/10 , C12N9/22 , C12Q1/6806 , C40B50/14 , C12Q1/686
CPC分类号: C12N15/1065 , B01J2219/00351 , B01J2219/00596 , B01J2219/00641 , C12N9/22 , C12N15/1017 , C12Q1/6806 , C12Q1/686 , C12Q2521/543 , C12Q2525/191 , C12Q2563/179 , C40B50/14 , C12Q2535/122 , C12Q2565/518
摘要: Presented herein are methods and compositions surface-based tagmentation. In particular embodiments, methods of preparing an immobilized library of fragmented and tagged DNA molecules on a solid surface are presented. In particular embodiments, the solid surface comprises immobilized transposomes in a dried format, suitable for reconstitution upon contact with liquid, such as a liquid sample.
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公开(公告)号:US20180149656A1
公开(公告)日:2018-05-31
申请号:US15826494
申请日:2017-11-29
IPC分类号: G01N33/574 , C12Q1/68 , G01N33/58
CPC分类号: G01N33/57488 , C07K2317/32 , C12Q1/68 , C12Q1/6834 , C12Q2521/543 , C12Q2525/173 , C12Q2565/518 , G01N33/54353 , G01N33/58 , C12Q2565/607
摘要: A multiplex-able, regeneratable nucleic-acid linked immunoassay method and system for the detection of a single specific, or multiple, soluble analytes in solution and regeneratable biosensor devices for same are described.
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公开(公告)号:US09920354B2
公开(公告)日:2018-03-20
申请号:US13328280
申请日:2011-12-16
CPC分类号: C12Q1/6806 , C12Q1/6844 , C12Q1/6851 , C12Q1/703 , C12Q1/706 , C12Q2565/518 , C12Q2545/101 , C12Q2537/143 , C12Q2561/113 , C12Q2545/113
摘要: The present invention is in the field of in-vitro diagnostics. Within this field, it provides the amplification of at least a first target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer. It further provides an analytical system comprising an internal control nucleic acid for qualitative and/or quantitative purposes and at least one external control nucleic acid in an aqueous buffer.
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公开(公告)号:US20170356056A1
公开(公告)日:2017-12-14
申请号:US15688783
申请日:2017-08-28
申请人: Mike J. Powell , Aiguo Zhang
发明人: Mike J. Powell , Aiguo Zhang
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6886 , C12Q1/6858 , C12Q2600/156 , C12Q2549/126 , C12Q2565/518 , C12Q2565/519
摘要: This invention provides methods and systems for detecting sequence variants in a sample nucleic acid. Methods include PNA clamping/PCR using primers with binding moieties followed by capture and detection of amplicons on a solid support. Systems include PCR reagents with primers having binding moieties, a PNA clamping probe, a solid support capable of capturing the PCR amplicons incorporating the binding moieties, and a detector device.
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公开(公告)号:US20170298422A1
公开(公告)日:2017-10-19
申请号:US15470635
申请日:2017-03-27
发明人: Chunxiao Song , Stephen R. Quake , Axel Brunger , Jiajie Diao
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6818 , C12Q1/6834 , C12Q2537/164 , C12Q2563/107 , C12Q2565/518
摘要: Provided herein is a method for analyzing genomic DNA. In some embodiments, the method may comprise labeling a genomic sample by adding a capture tag to the ends of the DNA molecules in the sample and labeling molecules that comprise hydroxymethylcytosine with a first fluorophore, immobilizing the labeled DNA molecules on a support, and imaging individual molecules of hydroxymethylated genomic DNA on the support.
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公开(公告)号:US20170247734A1
公开(公告)日:2017-08-31
申请号:US15453733
申请日:2017-03-08
发明人: Brian Christopher GODWIN , Priya SHANBHAG , Craig Elder MEALMAKER , Gianni Calogero FERRERI , Melinda PALMER , Shally Hsueh-Wen WANG
CPC分类号: C12P19/34 , C12Q1/6837 , C12Q1/6844 , C12Q1/6853 , C12Q1/6869 , C12Q2525/191 , C12Q2565/518 , C12Q2525/161 , C12Q2563/173 , C12Q2565/537
摘要: An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule.
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