公开(公告)号：US20150165440A1

公开(公告)日：2015-06-18

申请号：US14539822

申请日：2014-11-12

Applicant: STOKES BIO LIMITED

Inventor： Mark Davies ,  John Daly ,  Tara Dalton

IPC: B01L7/00 ,  G01N21/64

CPC classification number: B01L7/525 ,  B01L3/508 ,  B01L7/54 ,  B01L2300/0654 ,  B01L2300/0809 ,  B01L2300/1827 ,  B01L2400/0442 ,  B01L2400/0445 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2201/062

Abstract: An apparatus (1) is for DNA amplification with quantitative measurements. A biological sample is held in a cell (2) for the amplification, the cell (2) defining a single space within which the sample rotates. On one side a copper heater (3) is located to supply heat to the cell (2), and on the other side there is a cooling copper block (4) withdrawing heat from the cell. The locations of the heater (3) and the cooling block (4) generate a natural convection loop internally within the cell (2) without need for active cooling—the block (4) passively cooling by withdrawing heat from the direction of the heater (3). A detector (9, 27) captures readings in real time and a processor (10) generates an S-curve for change of sample emission with time. The S-curve (FIGS. 4 and 5) also includes a thermal cycle number corresponding to the time parameter, so that the S-curve is given in the traditional qPCR intensity vs. cycle number.

Abstract translation: 装置（1）用于通过定量测量进行DNA扩增。 将生物样品保持在用于扩增的细胞（2）中，细胞（2）限定样品旋转的单个空间。 在一侧，铜加热器（3）被定位成向电池（2）供热，另一侧有一个冷却铜块（4）从电池中取出热量。 加热器（3）和冷却块（4）的位置在电池（2）内部产生自然对流回路，而不需要主动冷却 - 通过从加热器的方向抽出热量来阻止块（4）的被动冷却（ 3）。 检测器（9,27）实时捕获读数，并且处理器（10）产生用于随时间改变样品发射的S曲线。 S曲线（图4和图5）还包括对应于时间参数的热循环数，使得S曲线以传统的qPCR强度对周期数给出。