公开(公告)号：US11931738B2

公开(公告)日：2024-03-19

申请号：US17045873

申请日：2018-11-22

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： John Lahmann ,  Pavel Kornilovich ,  Silam J Choy

IPC: B01L3/00

CPC classification number: B01L3/50273 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2300/0645 ,  B01L2400/0406 ,  B01L2400/0442

Abstract: An example method includes providing fluid to a chamber. The chamber feeds a first channel terminating at a first droplet ejector and a second channel terminating at a second droplet ejector. The method further includes sequencing ejection of droplets at the first droplet ejector and the second droplet ejector to induce negative pressure to provide a sequenced output flow of the fluid through the first channel to a first target microfluidic network and through the second channel to a second target microfluidic network, and controlling the first and second target microfluidic networks to perform an analytical process with the fluid.

公开(公告)号：US11896973B2

公开(公告)日：2024-02-13

申请号：US17211480

申请日：2021-03-24

Applicant: CYTONOME/ST, LLC

Inventor： Donald Francis Perrault, Jr. ,  Johnathan Charles Sharpe ,  Erin Koksal

IPC: B01L3/00 ,  G01N35/00 ,  G01N21/00 ,  G01N15/10

CPC classification number: B01L3/502738 ,  B01L3/502761 ,  G01N21/00 ,  G01N35/00 ,  B01L3/5027 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0622 ,  G01N2015/1081

Abstract: A microfluidic chip assembly having a plurality of microfluidic flow channels is provided. Each channel has a switching region. The microfluidic chip may further include at least one bubble jet actuator configured to generate a pressure pulse in the switching regions of the channels to selectively deflect particles in the flow. The bubble jet actuator may be configured as a blind chamber, as an operative non-through flow chamber and/or as a self-replenishment chamber. The bubble jet actuator may include a trapped air bubble. The bubble jet actuator may include a plurality of heating elements individually controlled for pre-nucleation warmup and/or for triggering vapor bubble nucleation.

公开(公告)号：US11802264B2

公开(公告)日：2023-10-31

申请号：US17067971

申请日：2020-10-12

Applicant: Berkeley Lights, Inc.

Inventor： Volker L. S. Kurz ,  Troy A. Lionberger ,  Eric K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/00 ,  C12M1/26 ,  G01N15/14

CPC classification number: C12M23/16 ,  B01L3/502761 ,  C12M23/20 ,  C12M33/12 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US11789015B2

公开(公告)日：2023-10-17

申请号：US16948074

申请日：2020-09-01

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt

IPC: G01N33/543 ,  B01L3/00 ,  B01F31/65 ,  B01F33/451 ,  B01F33/302 ,  B01F33/3031 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F31/65 ,  B01F33/3021 ,  B01F33/3031 ,  B01F33/451 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  G01N1/38 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/089 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575 ,  Y10T436/25625

Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

公开(公告)号：US11666910B2

公开(公告)日：2023-06-06

申请号：US17268394

申请日：2018-11-26

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Daryl E. Anderson ,  George H. Corrigan, III ,  Alexander Govyadinov

IPC: B01L7/00 ,  C12Q1/686 ,  B01L3/00

CPC classification number: B01L3/50273 ,  B01L7/525 ,  C12Q1/686 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/1827 ,  B01L2400/0442

Abstract: In one example in accordance with the present disclosure, a microfluidic device is described. The microfluidic device includes a reservoir to contain a first thermally expandable fluid, a first heater to heat the thermally expandable fluid in the reservoir, a channel extending from the reservoir and connected to the reservoir at a first opening, and a liquid volume obstructing the channel.

公开(公告)号：US20190210025A1

公开(公告)日：2019-07-11

申请号：US16099172

申请日：2016-07-26

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Chantelle DOMINGUE ,  Tod WOODFORD ,  Manish GIRI ,  Matthew David SMITH ,  George H CORRIGAN III ,  Masoud ZAVAREHI ,  Joshua M. YU

IPC: B01L3/00 ,  G01N15/10

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/5085 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0439 ,  B01L2400/0442 ,  G01N15/1031 ,  G01N15/1056 ,  G01N35/1065 ,  G01N2015/1006 ,  G01N2015/1062 ,  G01N2015/1081

Abstract: According to an example, a microfluidic apparatus may include a fluid slot and a foyer that is in fluid communication with the fluid slot via a channel having a relatively smaller width than the foyer. The microfluidic apparatus may also include an electrical sensor to measure a change in an electrical field caused by a particle of interest in a fluid passing through the channel from the fluid slot to the foyer, an actuator to apply pressure onto fluid contained in the foyer, and a controller to receive the measured change in the electrical field from the electrical sensor, determine, from the received change in the electrical field, an electrical signature of the particle of interest, and control the actuator to control movement of the particle of interest based upon the determined electrical signature of the particle of interest.

公开(公告)号：US20190022650A1

公开(公告)日：2019-01-24

申请号：US15792732

申请日：2017-10-24

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Steven Barcelo ,  Anita Rogacs ,  Viktor Shkolnikov ,  Milo Overbay

IPC: B01L3/00 ,  B01L9/00 ,  B29C39/00 ,  G01N21/65 ,  G01N21/64

CPC classification number: B01L3/502738 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/527 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0433 ,  B01L2400/0442 ,  B29C39/006 ,  G01N21/6486 ,  G01N21/658 ,  G01N2021/651

Abstract: A microfluidic chip may include a substrate, chamber supported by the substrate, a sacrificial material in the chamber, a spectroscopically active nano particle assembly anchored within the chamber by the sacrificial material and a fluid supply port connected to the chamber. Each spectroscopically active nano particle assembly may include a cluster of nanoparticles.

公开(公告)号：US20180298318A1

公开(公告)日：2018-10-18

申请号：US16010176

申请日：2018-06-15

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. Kurz ,  Troy A. Lionberger ,  Erik K. Sackmann ,  Kai W. Szeto ,  Paul M. Lebel ,  Brandon R. Bruhn ,  Keith J. Breinlinger ,  Eric D. Hobbs ,  Andrew W. McFarland ,  J. Tanner Nevill ,  Xiaohua Wang

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/26

CPC classification number: C12M23/16 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086 ,  C12M23/20 ,  C12M33/12

Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

公开(公告)号：US10078078B2

公开(公告)日：2018-09-18

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  G01N33/561 ,  B03C1/28 ,  C12N15/10 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US20180113068A1

公开(公告)日：2018-04-26

申请号：US15570958

申请日：2015-07-24

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Timothy L. Weber

IPC: G01N21/05 ,  G01N21/64 ,  G01N21/59 ,  G01N21/11

CPC classification number: G01N21/05 ,  B01L3/50273 ,  B01L2300/0816 ,  B01L2400/0442 ,  G01N21/11 ,  G01N21/59 ,  G01N21/6428 ,  G01N2021/0328 ,  G01N2021/0346 ,  G01N2021/6439 ,  G01N2021/7783 ,  G01N2021/7786 ,  G01N2201/0221 ,  G01N2201/024 ,  G01N2201/0873

Abstract: In one example, testing cells extend along a length of a slot. Each testing cell includes a microfluidic channel extending from the slot, a pump to move fluid from the slot into the channel, a discharge nozzle through which fluid exits the channel, a fluid discharger to discharge fluid from the channel through the nozzle and a photosensor. A light guide is provided to receive light from an external light source and is to serially transmit the light to the microfluidic channel of each of the plurality of testing cells.