公开(公告)号：US20230295703A1

公开(公告)日：2023-09-21

申请号：US18164453

申请日：2023-02-03

Applicant: STOKES BIO LTD.

Inventor： Mark B. DAVIES ,  Tara DALTON

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487

Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.

公开(公告)号：US20200283837A1

公开(公告)日：2020-09-10

申请号：US16812987

申请日：2020-03-09

Applicant: STOKES BIO LTD.

Inventor： Mark B. DAVIES ,  Tara DALTON

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.