公开(公告)号：US20210023561A1

公开(公告)日：2021-01-28

申请号：US16922265

申请日：2020-07-07

Applicant: STOKES BIO LTD.

Inventor： Mark DAVIES ,  Tara DALTON ,  Kieran CURRAN

IPC: B01L3/00 ,  B01F13/00 ,  B01F15/02 ,  F17D1/08 ,  C12Q1/686

Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisym metric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

公开(公告)号：US20200283837A1

公开(公告)日：2020-09-10

申请号：US16812987

申请日：2020-03-09

Applicant: STOKES BIO LTD.

Inventor： Mark B. DAVIES ,  Tara DALTON

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.

公开(公告)号：US20230295703A1

公开(公告)日：2023-09-21

申请号：US18164453

申请日：2023-02-03

Applicant: STOKES BIO LTD.

Inventor： Mark B. DAVIES ,  Tara DALTON

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487

Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.