公开(公告)号：US5925529A

公开(公告)日：1999-07-20

申请号：US483506

申请日：1995-06-07

申请人： Shaun R. Coughlin ,  Ji Chen ,  Harold Bernstein ,  Maki Ishii ,  Ling Wang ,  Mian Chen

发明人： Shaun R. Coughlin ,  Ji Chen ,  Harold Bernstein ,  Maki Ishii ,  Ling Wang ,  Mian Chen

IPC分类号： C07K14/705 ,  C12Q1/37 ,  G01N33/68 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/68 ,  C07K14/705 ,  C12Q1/37 ,  G01N2333/974

摘要： This invention relates to peptide ligand discovery and is particularly directed to a method for the discovery of agonists for membrane bound receptors. The inventive detection system involves the use of a "tethered" ligand for probing receptor binding. The general detection system includes a membrane, a membrane bound receptor, and a chimeric ligand presenting molecule. This chimeric protein forms the tethered ligand and in turn includes a membrane domain, a linker domain, a ligand domain, and a cleavable terminal domain. The "ligands" of the system are exposed by the addition of a specific peptidase that cleaves at the designated sequence. The sequence of the ligand that produces signal as a result of the interaction between the agonist and receptor can be then be isolated using sib selection.

摘要翻译： 本发明涉及肽配体发现，特别涉及发现膜结合受体激动剂的方法。 本发明的检测系统涉及使用“拴系”配体来探测受体结合。 一般检测系统包括膜，膜结合受体和嵌合配体呈递分子。 该嵌合蛋白形成栓系配体，并且又包括膜结构域，接头结构域，配体结构域和可裂解末端结构域。 通过加入以指定序列切割的特异性肽酶来暴露系统的“配体”。 由激动剂和受体之间的相互作用产生信号的配体的序列可以使用同位素选择进行分离。

公开(公告)号：US4414837A

公开(公告)日：1983-11-15

申请号：US348919

申请日：1982-02-16

申请人： James W. Bice ,  Charles L. Gravel ,  Harold Bernstein

发明人： James W. Bice ,  Charles L. Gravel ,  Harold Bernstein

IPC分类号： G01D18/00 ,  G01L1/22 ,  G01L9/06 ,  G01L25/00 ,  G01R17/10

CPC分类号： G01L1/225 ,  G01D18/00 ,  G01L25/00 ,  G01L9/065 ,  G01R17/105

摘要： There is disclosed an apparatus and a technique for shunt calibration of a Wheatstone bridge array independent of temperature. The structure involves a side completion half bridge array which has two temperature sensitive resistors as semiconductor strain gages forming one arm of the bridge and two temperature insensitive resistors forming the other arm of the bridge. An input voltage is applied to two opposite terminals of the bridge via equal span resistors and an output is taken between the common terminals of each of the bridge arms. A calibration resistor is positioned to shunt a span resistor and a temperature insensitive resistor to provide a calibrated output voltage of a magnitude independent of temperature.

摘要翻译： 公开了一种独立于温度的惠斯登电桥阵列的并联校准的装置和技术。 该结构涉及一个侧面完成半桥阵列，其具有两个温度敏感电阻器，作为形成桥的一个臂的半导体应变计和形成桥的另一个臂的两个温度不敏感电阻。 通过等距电阻将输入电压施加到桥的两个相对端子，并且在每个桥臂的公共端子之间采取输出。 定位电阻器用于分流跨度电阻器和温度不敏感电阻器，以提供与温度无关的幅度的校准输出电压。

公开(公告)号：US3951707A

公开(公告)日：1976-04-20

申请号：US460818

申请日：1974-04-15

申请人： Anthony D. Kurtz ,  Joseph R. Mallon ,  Harold Bernstein ,  Richard Alan Weber

发明人： Anthony D. Kurtz ,  Joseph R. Mallon ,  Harold Bernstein ,  Richard Alan Weber

IPC分类号： G01L9/00 ,  H01L21/58 ,  H01L41/22 ,  C23F1/04

CPC分类号： H01L24/83 ,  G01L9/0042 ,  H01L24/29 ,  H01L41/332 ,  H01L2224/2919 ,  H01L2224/8319 ,  H01L2224/8385 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01014 ,  H01L2924/01015 ,  H01L2924/01027 ,  H01L2924/01032 ,  H01L2924/01033 ,  H01L2924/01082 ,  H01L2924/0665 ,  H01L2924/07802 ,  H01L2924/14 ,  H01L2924/351 ,  Y10T29/42

摘要： A glass or other dielectric backed transducer structure is formed by utilizing a series of processes including at least one electrostatic bond. The processes enable one to bond a semiconductor wafer to a dielectric as a glass wafer. Then by selectively removing certain conductively semiconductor, one obtains a "thin ribbon" piezoresistive bridge secured to a thin glass wafer. The resultant structure is entirely unanticipated by the prior art.A glass part is also formed by electrostatically bonding a glass wafer to a semiconductor wafer, polishing the glass to a desired depth, masking the polished glass layer according to a desired pattern representative of the glass part, etching away all the glass except the desired pattern, and thence removing all the semiconductor.

摘要翻译： 通过利用包括至少一个静电键的一系列工艺形成玻璃或其它电介质的传感器结构。 这些工艺使得能够将半导体晶片与玻璃晶片的电介质接合。 然后通过选择性地去除某些导电半导体，获得固定到薄玻璃晶片的“薄带”压阻电桥。 所得到的结构完全是由现有技术无法预料的。

公开(公告)号：US20050260202A1

公开(公告)日：2005-11-24

申请号：US11084591

申请日：2005-03-17

申请人： Harold Bernstein ,  James Bristow

发明人： Harold Bernstein ,  James Bristow

IPC分类号： A61K39/395 ,  A61K48/00 ,  C07K16/28

CPC分类号： C07K16/28 ,  A61K35/12 ,  C12N5/0658 ,  C12N2501/50

摘要： The present invention is related to compositions and methods for expanding cell populations suitable for use as cardiac or skeletal muscle grafts. In particular, the present invention provides methods for regulation of cell cycle withdrawal and myoblast fusion during myogenesis.

摘要翻译： 本发明涉及用于扩增适合用作心脏或骨骼肌移植物的细胞群体的组合物和方法。 特别地，本发明提供了在肌发生期间调节细胞周期退出和成肌细胞融合的方法。