公开(公告)号：US20190192735A1

公开(公告)日：2019-06-27

申请号：US14001327

申请日：2012-02-27

申请人： Ryan C. Bailey ,  Brendan A. Harley ,  Teresa A. Martin ,  Steven R. Caliari

发明人： Ryan C. Bailey ,  Brendan A. Harley ,  Teresa A. Martin ,  Steven R. Caliari

IPC分类号： A61L27/38 ,  C12N5/00 ,  C08B37/00 ,  C12N5/077

CPC分类号： A61L27/38 ,  A61K35/12 ,  A61K35/32 ,  A61K38/1858 ,  A61K38/30 ,  A61L27/26 ,  A61L27/48 ,  A61L27/50 ,  A61L27/54 ,  A61L27/56 ,  A61L2430/02 ,  A61L2430/06 ,  A61L2430/20 ,  A61L2430/28 ,  A61L2430/30 ,  A61L2430/32 ,  A61L2430/40 ,  C08B37/0063 ,  C12N5/0062 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0657 ,  C12N5/0658 ,  C12N2533/54 ,  C08L89/06

摘要： The invention provides methods and compositions for making and using collagen-glycosaminoglycan three-dimensional scaffolds immobilized with biomolecules that are spatially and temporally patterned. The method comprises adding benzophenone to a collagen-glycosaminoglycan three dimensional scaffold in the dark; adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold (which can be done optionally in the dark or in the light); and exposing the collagen-2glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.

公开(公告)号：US09999638B2

公开(公告)日：2018-06-19

申请号：US15272797

申请日：2016-09-22

申请人： VBI Technologies, L.L.C.

发明人： Martin P. Vacanti ,  Charles A. Vacanti

IPC分类号： A61K35/407 ,  C12N5/074 ,  C12N5/071 ,  C12N5/0797 ,  C12N5/0789 ,  C12N5/0775 ,  C12N5/079 ,  C12N5/077 ,  A61K35/22 ,  A61K35/28 ,  A61K35/30 ,  A61K35/34 ,  A61K35/42 ,  A61K35/12

CPC分类号： A61K35/407 ,  A61K35/22 ,  A61K35/28 ,  A61K35/30 ,  A61K35/34 ,  A61K35/42 ,  A61K2035/124 ,  C12N5/0607 ,  C12N5/0614 ,  C12N5/0618 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0652 ,  C12N5/0658 ,  C12N5/0668 ,  C12N5/0669 ,  C12N5/0672 ,  C12N5/0688 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/392 ,  Y10T436/101666 ,  Y10T436/25375

摘要： Subpopulations of spore-like cells expressing specific cell surface and gene expression markers are provided. In one embodiment, the cells express at least one cell surface or gene expression marker selected from the group consisting of Oct4, nanog, Zfp296, cripto, Gdf3, UtF1, Ecat1, Esg1, Sox2, Pax6, nestin, SCA-1, CD29, CD34, CD90, B1 integrin, cKit, SP-C, CC10, SF1, DAX1, and SCG10. Also provided are methods for purifying a subpopulation of spore-like cells of interest from a population of spore-like cells, and methods for inducing differentiation of the isolated spore-like cells into cells of endodermal, mesodermal or ectodermal origin. The spore-like cells can be used to generate cells originating from all three germ layers and can be used to treat a patient who has a deficiency of functional cells in any of a wide variety of tissues, including the retina, intestine, bladder, kidney, liver, lung, nervous system, or endocrine system.

公开(公告)号：US09994812B2

公开(公告)日：2018-06-12

申请号：US14390490

申请日：2013-03-15

申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

发明人： Deok-Ho Kim ,  Michael Laflamme ,  Charles Murry ,  Kshitiz Gupta ,  Hyok Yoo ,  Alex Jiao

IPC分类号： A61K48/00 ,  C12N5/0735 ,  A61L27/14 ,  C12M3/00 ,  C12M1/12 ,  A61L27/18 ,  A61L27/38 ,  A61L27/50 ,  B01L3/00 ,  C12N5/077 ,  C12N5/074

CPC分类号： C12N5/0606 ,  A61K48/00 ,  A61L27/14 ,  A61L27/18 ,  A61L27/3826 ,  A61L27/3834 ,  A61L27/3873 ,  A61L27/50 ,  A61L2400/12 ,  A61L2400/18 ,  B01L3/5085 ,  B01L2300/0851 ,  B01L2300/0896 ,  B01L2400/086 ,  C12M21/08 ,  C12M25/14 ,  C12N5/0657 ,  C12N5/0658 ,  C12N5/0696 ,  C12N2503/02 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2535/00 ,  C08L67/04

摘要： The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition. The nanotextured substrate/cell constructs provide a platform for screening to predict the effect of test agents or drugs on, for example, human cardiac tissue, including patient-derived tissue, or for the identification of agents that effect various cardiac functional parameters.

公开(公告)号：US20180155673A1

公开(公告)日：2018-06-07

申请号：US15736035

申请日：2016-06-24

申请人： Tokyo Women's Medical University

发明人： Tatsuya Shimizu ,  Yuji Haraguchi ,  Yuki Kagawa ,  Katsuhisa Sakaguchi

IPC分类号： C12N1/12 ,  C12N5/077

CPC分类号： C12N1/12 ,  A61L27/3604 ,  A61L27/3895 ,  C12N5/0657 ,  C12N5/0658 ,  C12N2502/70 ,  C12N2529/10 ,  C12N2533/90

摘要： An object of the present invention is to obtain a thicker animal cell composition by a simple and less expensive method. Namely, an object of the present invention is to provide a method for culturing a thicker animal cell composition by eliminating the hypoxia associated with animal cell compositions, a method for producing an animal cell composition containing unicellular algae, and an animal cell composition.The present invention provides a method for culturing an animal cell composition in a culture medium in the presence of unicellular algae and under exposure to light. According to the method of the present invention, oxygen can be continuously supplied in the culture medium, cell damage is alleviated, and a thicker cell composition can be obtained in the absence of a capillary network.

公开(公告)号：US20180024119A1

公开(公告)日：2018-01-25

申请号：US15648319

申请日：2017-07-12

申请人： Emulate Inc.

发明人： Daniel Levner ,  Kyung Jin Jang ,  Jacob Fraser ,  Jordan Kerns ,  Antonio Varone ,  Dongeun Huh

IPC分类号： G01N33/50 ,  C12N5/071 ,  C12N5/00

CPC分类号： G01N33/5032 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12N5/0018 ,  C12N5/0068 ,  C12N5/0658 ,  C12N5/067 ,  C12N2500/32 ,  C12N2521/00 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90 ,  C12N2535/10 ,  C12N2537/10 ,  G01N33/5014 ,  G01N33/5044

摘要： Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

公开(公告)号：US09862929B2

公开(公告)日：2018-01-09

申请号：US14357982

申请日：2012-08-17

申请人： Hidetoshi Sakurai ,  Akihito Tanaka ,  Knut Woltjen ,  Makoto Ikeya

发明人： Hidetoshi Sakurai ,  Akihito Tanaka ,  Knut Woltjen ,  Makoto Ikeya

IPC分类号： C12N5/00 ,  C12N5/077 ,  C12N15/85 ,  G01N33/50

CPC分类号： C12N5/0658 ,  C12N15/85 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2800/22 ,  G01N33/5061

摘要： The present invention provides a method of producing a skeletal muscle cell from a pluripotent stem cell, which includes a step of expressing one or more exogenous factors selected from MyoD, Myf5 and nucleic acids encoding them on a pluripotent stem cell.

公开(公告)号：US20170252354A1

公开(公告)日：2017-09-07

申请号：US15498293

申请日：2017-04-26

申请人： The Board of Trustees of the Leland Stanford Junior University

发明人： Helen M. Blau ,  Andrew Tri Van Ho ,  Adelaida R. Palla

IPC分类号： A61K31/5575 ,  C12N5/077 ,  A61K35/34

CPC分类号： A61K31/5575 ,  A61K35/34 ,  C12N5/0658 ,  C12N2501/02 ,  A61K2300/00

摘要： Provided herein are compositions, methods, and kits for proliferating muscle cells by exposing the muscle cells to a prostaglandin E2 (PGE2) compound or compound that activates PGE2 signaling. Also provided are methods for regenerating muscle in a subject suffering from muscular atrophy, dystrophy, and/or injury by administering a PGE2 compound alone or in combination with isolated muscle cells. The PGE2 compound in combination with the isolated muscle cells can be administered prophylactically to prevent a muscle disease or condition.

公开(公告)号：US09556418B2

公开(公告)日：2017-01-31

申请号：US13765104

申请日：2013-02-12

申请人： Wake Forest University Health Sciences

发明人： George J. Christ ,  Benjamin T. Corona

IPC分类号： C12N5/071 ,  A61F2/08 ,  A61K35/34 ,  C12N5/077 ,  C12M1/42 ,  A61K35/12

CPC分类号： A61L27/3895 ,  A61F2/08 ,  A61F2002/0894 ,  A61K35/12 ,  A61K35/34 ,  A61L27/3826 ,  A61L27/3873 ,  C12M35/02 ,  C12N5/0658 ,  C12N5/0697 ,  C12N2527/00 ,  C12N2533/54 ,  C12N2533/90

摘要： Provided herein are methods of culturing organized skeletal muscle tissue from precursor muscle cells by cyclically stretching and relaxing said muscle cells on a support in vitro for a time sufficient to produce said organized skeletal muscle tissue, including reseeding said organized skeletal muscle tissue by contacting additional precursor muscle cells to said organized skeletal muscle tissue on said solid support, and then repeating said step of cyclically stretching and relaxing said muscle cells in said support in vitro for time sufficient to enhance the density (i.e., increased number of nuclei and/or number of multinucleated cells) of said organized skeletal muscle tissue on said support.

摘要翻译： 本文提供的方法是通过在体外在载体上循环拉伸和松弛所述肌肉细胞足以产生所述有组织的骨骼肌组织的时间，包括通过使另外的前体接触来重新接合所述组织的骨骼肌组织，从前体肌细胞培养有组织的骨骼肌组织 将肌细胞移动到所述固体支持物上的所述有组织的骨骼肌组织，然后重复所述在体外循环拉伸和松弛所述支持体中所述肌肉细胞的时间足以提高密度（即增加的细胞核数量和/或数量） 所述组织的骨骼肌组织在所述支持体上的多核细胞）。

公开(公告)号：US09506025B2

公开(公告)日：2016-11-29

申请号：US12429385

申请日：2009-04-24

申请人： James Yoo ,  Joel Stitzel ,  Anthony Atala ,  George Christ

发明人： James Yoo ,  Joel Stitzel ,  Anthony Atala ,  George Christ

IPC分类号： A61F2/08 ,  C12M1/42 ,  A61L27/38 ,  C12M3/00 ,  C12N5/077

CPC分类号： A61F2/08 ,  A61F2002/0894 ,  A61L27/3604 ,  A61L27/367 ,  A61L27/3683 ,  A61L27/3691 ,  A61L27/3826 ,  A61L27/3895 ,  A61L2430/30 ,  A61L2430/34 ,  C12M21/08 ,  C12M35/04 ,  C12N5/0658 ,  C12N2527/00 ,  C12N2539/00

摘要： A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

摘要翻译： 从前体肌细胞体外产生有组织的骨骼肌组织的方法包括：（a）在组织培养基中的支持体上提供前体肌细胞; 然后（b）在第一时间段期间沿着第一轴线循环地拉伸和松弛支撑件至少两次; 然后（c）任选地，但优选在第二时间段内将载体保持在基本上静止的位置; 然后（d）重复步骤（b）和（c）足以增强在载体上形成的组织的功能性和/或从前体肌细胞在固体支持物上产生有组织的骨骼肌组织的次数。

公开(公告)号：US09493735B2

公开(公告)日：2016-11-15

申请号：US11279671

申请日：2006-04-13

申请人： James Yoo ,  Joel Stitzel ,  Anthony Atala ,  George Christ

发明人： James Yoo ,  Joel Stitzel ,  Anthony Atala ,  George Christ

IPC分类号： C12M3/00 ,  C12M1/42 ,  A61L27/38 ,  C12N5/077

CPC分类号： A61F2/08 ,  A61F2002/0894 ,  A61L27/3604 ,  A61L27/367 ,  A61L27/3683 ,  A61L27/3691 ,  A61L27/3826 ,  A61L27/3895 ,  A61L2430/30 ,  A61L2430/34 ,  C12M21/08 ,  C12M35/04 ,  C12N5/0658 ,  C12N2527/00 ,  C12N2539/00

摘要： A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.

摘要翻译： 从前体肌细胞体外产生有组织的骨骼肌组织的方法包括：（a）在组织培养基中的支持体上提供前体肌细胞; 然后（b）在第一时间段期间沿着第一轴线循环地拉伸和松弛支撑件至少两次; 然后（c）任选地，但优选在第二时间段内将载体保持在基本上静止的位置; 然后（d）重复步骤（b）和（c）足以增强在载体上形成的组织的功能性和/或从前体肌细胞在固体支持物上产生有组织的骨骼肌组织的次数。