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1.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US08480867B2
公开(公告)日：2013-07-09
申请号：US13135848
申请日：2011-07-15
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： Apparatus are disclosed for measuring substrate content present in a specimen that is applied to a biosensor, where the apparatus include an input for receiving an electrical signal from the biosensor; and a circuit coupled to the input to detect the electrical signal to determine whether an amount of the specimen needed for specimen detection has been supplied to the biosensor; where the apparatus measures the substrate content included in the specimen when the circuit determines that an amount of the specimen needed for specimen detection has been supplied to the biosensor, and the apparatus is prevented from measuring the substrate content included in the specimen when the circuit determines that the specimen has not been sufficiently supplied to the biosensor.
摘要翻译： 公开了用于测量施加到生物传感器的样本中存在的底物含量的装置，其中装置包括用于从生物传感器接收电信号的输入端; 以及电路，其耦合到所述输入端以检测所述电信号，以确定是否向所述生物传感器提供了所述样本检测所需的样本量; 其中当电路确定样本检测所需的样本量已被提供给生物传感器时，该装置测量包含在样本中的基底含量，并且当电路确定时检测装置被测量包含在样本中的基底含量 样品没有被充分供应给生物传感器。
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2.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US08475646B2
公开(公告)日：2013-07-02
申请号：US13135816
申请日：2011-07-15
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： Methods are disclosed for measuring substrate content in a specimen using a biosensor that includes two pairs of electrodes in a specimen supply path, where the methods include the steps of: (a) a first detecting step for detecting an electrical change in the first pair of electrodes; (b) a second detecting step for detecting an electrical change in the second pair of electrodes after the first detecting step; (c) a judging step for judging a shortage of specimen amount that is needed for measurement, when an electrical change is not detected in the second detecting step within a prescribed period of time after the first detecting step; (d) an indicating step for indicating a shortage of the specimen amount; and (e) a stopping step for stopping measurement of substrate content in the specimen once the shortage of specimen amount is indicated in the indicating step.
摘要翻译： 公开了用于使用在样本供应路径中包括两对电极的生物传感器来测量样品中的基底含量的方法，其中所述方法包括以下步骤：（a）第一检测步骤，用于检测第一对中的电变化 电极 （b）第二检测步骤，用于在第一检测步骤之后检测第二对电极中的电变化; （c）当在第一检测步骤之后的规定时间内没有在第二检测步骤中检测到电变化时，判断测量所需的试样量的不足的判断步骤; （d）指示样本量不足的指示步骤; 和（e）一旦在指示步骤中指示了样本量不足，则停止步骤，用于停止测量样本中的基底含量。
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3.发明授权公开(公告)号：US08349157B2
公开(公告)日：2013-01-08
申请号：US13490553
申请日：2012-06-07
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
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4.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US08025780B2
公开(公告)日：2011-09-27
申请号：US12322684
申请日：2009-02-05
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译： 公开了一种生物传感器，其包括支撑件; 由导电材料如贵金属（例如金或钯）和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。 可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。
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5.发明申请Biosensor, thin film electrode forming method, quantification apparatus, and quantificaion method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US20090152111A1
公开(公告)日：2009-06-18
申请号：US12322684
申请日：2009-02-05
IPC分类号： C12M1/00
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9露出的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。
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6.发明授权Biosensor, thin film electrode forming method, quantification apparatus, and quantification method 有权生物传感器，薄膜电极形成方法，定量装置和定量方法公开(公告)号：US08142629B2
公开(公告)日：2012-03-27
申请号：US12802573
申请日：2010-06-09
IPC分类号： G01N27/327
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。
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7.发明申请公开(公告)号：US20110272296A1
公开(公告)日：2011-11-10
申请号：US13135816
申请日：2011-07-15
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
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8.发明申请公开(公告)号：US20110147234A1
公开(公告)日：2011-06-23
申请号：US12930957
申请日：2011-01-20
IPC分类号： G01N27/416
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
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公开(公告)号：US6004441A
公开(公告)日：1999-12-21
申请号：US889929
申请日：1997-07-10
IPC分类号： G01N27/327 , G01N27/403 , G01N33/487 , G01N33/52 , G01N27/26
CPC分类号： G01N27/3272 , G01N33/525 , Y10T156/1082
摘要： A biosensor is fabricated by forming a metal film over the surface of a substrate through evaporation, sputtering or by gluing a metal foil, then splitting the metal film into three regions, and forming a reagent layer on an area of measuring electrode and two counter-electrodes for placing a liquid sample on by providing a cover disposed over the splitted metal film. As neither of the electrodes are formed through conventional printing technology, the electrodes can be formed without dispersion. As a result, a precision biosensor having excellent response characteristics is implemented.
摘要翻译： 通过蒸发，溅射或粘合金属箔在基板的表面上形成金属膜，然后将金属膜分割成三个区域，并在测量电极的区域上形成试剂层， 用于通过提供设置在分离的金属膜上的盖来放置液体样品的电极。 由于既不通过常规印刷技术形成电极，也可以不分散地形成电极。 结果，实现了具有优异的响应特性的精密生物传感器。
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10.发明申请BIOSENSOR, THIN FILM ELECTRODE FORMING METHOD, QUANTIFICATION APPARATUS, AND QUANTIFICATION METHOD 有权生物传感器，薄膜电极形成方法，量化装置和定量方法公开(公告)号：US20120251708A1
公开(公告)日：2012-10-04
申请号：US13490553
申请日：2012-06-07
CPC分类号： G01N27/3272 , C12Q1/001 , G01N33/48771 , Y10T29/49002
摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
摘要翻译： 公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。 可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。
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