公开(公告)号：US5032514A

公开(公告)日：1991-07-16

申请号：US229598

申请日：1988-08-08

申请人： Stephen Anderson ,  Robert A. Lazarus ,  Harvey I. Miller ,  R. Kevin Stafford

发明人： Stephen Anderson ,  Robert A. Lazarus ,  Harvey I. Miller ,  R. Kevin Stafford

IPC分类号： C12N9/04 ,  C12P7/60

CPC分类号： C12N9/0006 ,  C12P7/60

摘要： In recombinant microorganisms which were rendered capable of converting 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG) by transfer of genetic material, the secondary metabolites and metabolic pathways leading to the metabolic diversion of 2-KLG and 2,5-DKG were determined, and the diversion of 2-KLG to L-iodonic acid (IA) or of 2,5-DKG to 5-keto-D-gluconate (5-KDH) was blocked.

摘要翻译： 在通过遗传物质转移能够将2,5-二酮-D-葡糖酸（2,5-DKG）转化为2-酮-L-古洛糖酸（2-KLG）的重组微生物中，次级代谢物和 确定导致2-KLG和2,5-DKG代谢转移的代谢途径，并且将2-KLG转移到L-碘酸（IA）或2,5-DKG转化为5-酮-D-葡萄糖酸盐 （5-KDH）。