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    Host transformed with yeast gene and ubiquitin/polypeptide fusions
    2.
    发明授权
    Host transformed with yeast gene and ubiquitin/polypeptide fusions 失效
    宿主用酵母基因和泛素/多肽融合物转化

    公开(公告)号：US5262322A

    公开(公告)日：1993-11-16

    申请号：US694522

    申请日：1991-04-30

    申请人： Chung-Cheng Liu ,  Harvey I. Miller

    发明人： Chung-Cheng Liu ,  Harvey I. Miller

    IPC分类号： C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C12N15/57 ,  C12N1/21

    CPC分类号： C07K14/00 ,  C07K14/64 ,  C12N9/60 ,  C07K2319/036 ,  C07K2319/75 ,  C07K2319/95

    摘要： A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

    摘要翻译： 提供泛蛋白水解酶，其基于组合物中总蛋白质的重量具有至少70％的纯度至少70％的纯度。 还提供了编码泛素水解酶的DNA序列，以及用于其重组生产的表达系统。 提供了从真核生物纯化泛素水解酶的方法，并且其用于在其N-末端用泛素回收任何无融合物的所需多肽。

    Polynucleotides encoding .alpha.2-3 neuraminidase
    3.
    发明授权
    Polynucleotides encoding .alpha.2-3 neuraminidase 失效
    编码α2-3神经氨酸酶的多核苷酸

    公开(公告)号：US5591839A

    公开(公告)日：1997-01-07

    申请号：US294477

    申请日：1994-08-18

    申请人： Harvey I. Miller ,  John C. Klock ,  Christopher M. Starr

    发明人： Harvey I. Miller ,  John C. Klock ,  Christopher M. Starr

    IPC分类号： C12N9/24 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Y302/01018 ,  C12N9/2402

    摘要： Polynucleotide sequences encoding enzymes that possess .alpha.2-3 neuraminidase activity are provided. Of particular interest are polynucleotide sequences encoding an enzyme having .alpha.2-3 neuraminidase activity and naturally produced by the bacteria Streptococcus pneumoniae. Recombinant DNA expression of enzymes possessing .alpha.2-3 specific neuraminidase activity is also described, including methods, recombinant host cells and a genetic construction. The invention also provides a purified enzyme having .alpha.2-3 neuraminidase activity from Streptococcus pneumoniae, wherein the enzyme is isolated from S. pneumoniea cultures. Another aspect of this invention is to provide methods of isolating genes encoding enzymes having neuraminidase activity, particularly .alpha.2-3 neuraminidase activity. The gene isolation methods of the invention comprise the step of labeling a hybridization probe derived from the neuraminidase coding portion of plasmid pND-1. The hybridization probe may then be used to isolate homologous genes encoding enzymes having the desired enzymatic activity.

    摘要翻译： 提供了编码具有α2-3神经氨酸酶活性的酶的多核苷酸序列。 特别感兴趣的是编码具有α2-3神经氨酸酶活性并由细菌肺炎链球菌天然产生的酶的多核苷酸序列。 还描述了具有α2-3特异性神经氨酸酶活性的酶的重组DNA表达，包括方法，重组宿主细胞和遗传构建。 本发明还提供了具有来自肺炎链球菌的α2-3神经氨酸酶活性的纯化酶，其中所述酶从肺炎链球菌培养物中分离。 本发明的另一方面是提供分离编码具有神经氨酸酶活性的酶的基因，特别是α2-3神经氨酸酶活性的方法。 本发明的基因分离方法包括标记衍生自质粒pND-1的神经氨酸酶编码部分的杂交探针的步骤。 然后可以使用杂交探针来分离编码具有所需酶活性的酶的同源基因。

    Balanced inducible transcription system
    4.
    发明授权
    Balanced inducible transcription system 失效
    平衡诱导型转录系统

    公开(公告)号：US5266474A

    公开(公告)日：1993-11-30

    申请号：US719056

    申请日：1991-06-21

    申请人： Harvey I. Miller

    发明人： Harvey I. Miller

    IPC分类号： C07K14/61 ,  C12N1/21 ,  C12N9/72 ,  C12N15/11 ,  C12N15/67 ,  C12N15/70 ,  C12N15/85 ,  C12N15/00 ,  C12N9/64

    CPC分类号： C12N9/6459 ,  C07K14/61 ,  C12N15/11 ,  C12N15/67 ,  C12N15/70 ,  C12N15/85 ,  C12Y304/21069 ,  C12N2830/002 ,  C12N2830/55

    摘要： A balanced constitutive inducible transcriptional control system is provided to facilitate the expression of polypeptides incompatible with recombinant host cells. Use of this system has, for the first time, made it possible to produce mature, unglycosylated human tissue plasminogen activator in prokaryotes which is water soluble and fully enzymatically active. In accordance with this invention, t-PA is produced by recombinant bacterial host cell culture in commercially significant amounts without in vitro renaturation and processing.

    摘要翻译： 提供平衡的组成型诱导转录控制系统以促进与重组宿主细胞不相容的多肽的表达。 该系统的使用首次使得可以在水溶性和完全酶活性的原核生物中产生成熟的，未糖基化的人组织纤溶酶原激活剂。 根据本发明，t-PA通过重组细菌宿主细胞培养以商业上显着的量生产，而无需体外复性和加工。