公开(公告)号：US20170045525A1

公开(公告)日：2017-02-16

申请号：US15116661

申请日：2015-02-24

Applicant: THE UNIVERSITY OF TOKYO

Inventor： Yasuteru URANO ,  Mako KAMIYA ,  Keitaro UMEZAWA ,  Masafumi YOSHIDA

IPC: G01N33/68 ,  C09K11/07 ,  C09B19/00 ,  G01N33/58 ,  G01N21/64 ,  C09B11/24

CPC classification number: G01N33/68 ,  C07F7/081 ,  C07F7/0812 ,  C09B11/08 ,  C09B11/24 ,  C09B11/28 ,  C09B19/00 ,  C09K11/06 ,  C09K11/07 ,  C09K2211/1007 ,  C09K2211/1033 ,  C09K2211/1055 ,  C09K2211/1088 ,  C09K2211/1096 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/582 ,  G01N33/6815 ,  G01N2021/6439 ,  G01N2021/6441

Abstract: [Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a —SH group; a detection method using said fluorescent probe; and a detection kit containing said probe.[Solution] A fluorescent probe for detecting a compound containing an —SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(Ra)(Rb), Ge(Ra)(Rb), Sn(Ra)(Rb), C(Ra)(Rb), or O (wherein Ra and Rb each independently represent a hydrogen atom or alkyl group); R1 represents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; R2 represents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; R3 and R4 each independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halogen atom, or an alkyl group, sulfo group, carboxyl group, ester group, amide group and azide group, each of which may be optionally substituted; R5, R6, R7 and R8 each independently represent a hydrogen atom or alkyl group, wherein, R5 or R6, respectively together with R3, may form a ring structure including the nitrogen atoms bonded thereto, or R7 or R8, respectively together with R4, may form a ring structure including the nitrogen atoms bonded thereto).

Abstract translation: [解决方案]用于检测含有-SH基团的化合物的荧光探针，其中所述荧光探针包含由式（I）表示的化合物或其盐（在该式中，X表示Si（Ra）（Rb），Ge Ra）（Rb），Sn（Ra）（Rb），C（Ra）（Rb）或O（其中Ra和Rb各自独立地表示氢原子或烷基）; R1表示氢原子或1-4 相同或不同的取代基，其独立地选自氰基，烷基，羧基，酯基，烷氧基，酰胺基和叠氮基，其各自可以被任选取代; R 2表示氢原子，卤素原子 ，羟基，氰基或烷基，炔基，烷氧基，芳基或杂芳基，其各自可以被任选取代; R 3和R 4各自独立地表示氢原子或1-3个相同或不同的独立选择的取代基 由羟基，卤素原子或烷基基团组成的组 p，磺基，羧基，酯基，酰胺基和叠氮基，其各自可以任选被取代; R 5，R 6，R 7和R 8各自独立地表示氢原子或烷基，其中，R 5或R 6分别与R 3一起形成包含与其结合的氮原子的环结构，或者R 7或R 8与R 4， 可以形成包括与其结合的氮原子的环结构）。