公开(公告)号：US20180112260A1

公开(公告)日：2018-04-26

申请号：US15821373

申请日：2017-11-22

Applicant: TOPPAN PRINTING CO., LTD. ,  TOKYO INSTITUTE OF TECHNOLOGY

Inventor： Shiro KITANO ,  Atsushi MARUYAMA ,  Naohiko SHIMADA

IPC: C12Q1/6832 ,  C12Q1/6869 ,  C12Q1/6827 ,  C08B37/02 ,  C08G73/02

CPC classification number: C12Q1/6832 ,  C08B37/0021 ,  C08G73/02 ,  C12N15/09 ,  C12Q1/25 ,  C12Q1/68 ,  C12Q1/6823 ,  C12Q1/6827 ,  C12Q1/6869 ,  G01N33/50 ,  G01N33/53 ,  C12Q2521/345 ,  C12Q2527/125

Abstract: The present invention relates to a method for detecting a target nucleic acid using a catalytic nucleic acid, which can improve the reaction efficiency of a catalytic nucleic acid and achieve continuous reaction under isothermal conditions. The method for detecting a target nucleic acid includes a reaction step to obtain a first nucleic acid product by hybridizing a first nucleic acid which is a substrate to a first binding site of a catalytic nucleic acid equipped with an active site having a catalytic action; and a detection step for detecting at least one of the first nucleic acid product or unreacted first nucleic acids, wherein, the hybridization is carried out in the presence of a cationic comb-type polymer.

公开(公告)号：US20240200025A1

公开(公告)日：2024-06-20

申请号：US18574611

申请日：2022-06-27

Applicant: NOF CORPORATION ,  TOKYO INSTITUTE OF TECHNOLOGY

Inventor： Ryutaro IMAMURA ,  Tomoyuki OHTAKE ,  Atsushi MARUYAMA ,  Naohiko SHIMADA

IPC: C12N5/00

CPC classification number: C12N5/0081 ,  C12N2509/00

Abstract: The present invention provides a cell detachment agent for detaching a cell to be separated from a separation carrier bound to the cell to be separated, wherein the above-mentioned cell detachment agent contains a bindable ureido group-containing polymer having a side chain structure represented by the following formula (1) and having a separation carrier-binding site capable of binding to the above-mentioned separation carrier at a side chain or terminal thereof, and the above-mentioned bindable ureido group-containing polymer has an upper critical solution temperature (UCST) of 4 to 40° C.:


—NH—(C═O)—NH2  (1)