公开(公告)号：US12109262B2

公开(公告)日：2024-10-08

申请号：US17805419

申请日：2022-06-03

Applicant: Osaka University

Inventor： Takeshi Kobayashi ,  Yuta Kanai

IPC: A61K39/15 ,  A61P31/14 ,  C07K14/14 ,  C12N15/09 ,  C12N15/86

CPC classification number: A61K39/15 ,  A61P31/14 ,  C07K14/14 ,  C12N15/09 ,  C12N15/86

Abstract: Provided is a method for producing an artificial recombinant virus of the family Reoviridae, the method comprising the steps of:



(1) introducing a FAST protein expression vector and/or a capping enzyme expression vector into host cells;
(2) introducing a vector containing expression cassettes for individual RNA genome segments of a virus or introducing a set of single-stranded RNA transcripts from the expression cassettes into host cells; and
(3) culturing the host cells.




The method of the present invention allows more efficient production of an artificial recombinant virus of the family Reoviridae as compared with conventional methods and allows artificial recombinant rotavirus production without using a helper virus.

公开(公告)号：US12098389B2

公开(公告)日：2024-09-24

申请号：US17844757

申请日：2022-06-21

Applicant: KEIO UNIVERSITY

Inventor： Minoru Ko

IPC: C12N5/071 ,  C12N5/077 ,  C12N5/0793 ,  C12N15/09 ,  C12N15/113

CPC classification number: C12N5/0658 ,  C12N5/0619 ,  C12N5/067 ,  C12N15/09 ,  C12N15/113 ,  C12N2310/14 ,  C12N2506/02

Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.

公开(公告)号：US12091701B2

公开(公告)日：2024-09-17

申请号：US16089593

申请日：2017-03-28

Applicant: KARYDO THERAPEUTIX, INC.

Inventor： Narutoku Sato

IPC: G01N33/48 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  G16B20/00

CPC classification number: C12Q1/025 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/5008 ,  G01N33/53 ,  G01N33/6845 ,  G01N33/6893 ,  G01N2500/04 ,  G01N2500/10 ,  G01N2800/347 ,  G01N2800/52 ,  G01N2800/56 ,  G01N2800/60 ,  G01N2800/70 ,  G16B20/00

Abstract: This invention provides a device for screening a candidate substance for an active ingredient to prevent or treat declined kidney function etc. A screening device 1 for screening a candidate substance for an active ingredient to prevent or treat declined kidney function etc. comprises a first measurement value obtaining unit 11 for obtaining a measurement value of a kidney function prediction marker protein and/or a measurement value of mRNA of the protein in a test-substance-treated specimen, and a candidate substance determination unit 14 for determining that the test substance is a candidate substance for the active ingredient based on the measurement value(s) obtained by the first measurement value obtaining unit 11.

公开(公告)号：US12091671B2

公开(公告)日：2024-09-17

申请号：US17256441

申请日：2019-06-28

Applicant: Board of Trustees of Michigan State University

Inventor： Kyung-Hwan Han ,  Jong Hee Im

IPC: C12N15/82 ,  C07K1/107 ,  C07K14/415 ,  C12N15/09 ,  C12N15/10

CPC classification number: C12N15/8261 ,  C07K1/1075 ,  C07K14/415 ,  C12N15/102 ,  C12N15/09

Abstract: Described herein are modified MYB46 transcription factors that are more stable and less prone to degradation than corresponding unmodified MYB46 transcription factors. Expression of the modified MYB46 transcription factors within plants improves the structural strength, increases biomass, and enhances fiber strength of the plants.

公开(公告)号：US20240279742A1

公开(公告)日：2024-08-22

申请号：US18361440

申请日：2023-07-28

Applicant: TORAY INDUSTRIES, INC. ,  NATIONAL CANCER CENTER

Inventor： Junpei KAWAUCHI ,  Hitoshi NOBUMASA ,  Satoko KOZONO ,  Satoshi KONDOU ,  Hiroko SUDO ,  Atsushi OCHIAI ,  Motohiro KOJIMA

IPC: C12Q1/6886 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00

CPC classification number: C12Q1/6886 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  C12Q2600/158 ,  C12Q2600/178

Abstract: The present invention provides a kit or device for the detection of biliary tract cancer, and a method for detecting biliary tract cancer. The present invention relates to a kit or device for the detection of biliary tract cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting biliary tract cancer, comprising measuring the miRNA in vitro.

公开(公告)号：US12060616B2

公开(公告)日：2024-08-13

申请号：US16319024

申请日：2017-07-18

Applicant: OTSUKA PHARMACEUTICAL CO., LTD.

Inventor： Yoshihide Miyazaki ,  Yukari Umehara ,  Osamu Kosaka ,  Kimiyoshi Sudou

IPC: C12Q1/6886 ,  C12Q1/68 ,  C12Q1/686 ,  G16H50/20 ,  C12N15/09

CPC classification number: C12Q1/6886 ,  C12Q1/68 ,  C12Q1/686 ,  G16H50/20 ,  C12N15/09 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y102/01009

Abstract: Provided are a method for assisting in determining the hematological stage of childhood acute lymphoblastic leukemia (child ALL), and an in-vitro diagnostic pharmaceutical product usable in the method. The method for assisting in determining the hematological stage of child ALL comprises the steps of (1) obtaining the mRNA level of Wilms' tumor-1 gene (WT1) in at least one of the biological samples of peripheral blood and bone marrow fluid from a test subject; (2) obtaining the GAPDH mRNA level in the biological sample; and (3) calculating an index value necessary for assisting in the determination based on the ratio of the WT1 mRNA level obtained in step (1) to the GAPDH mRNA level obtained in step (2).

公开(公告)号：US12060561B2

公开(公告)日：2024-08-13

申请号：US16636622

申请日：2018-08-01

Applicant: CEVEC Pharmaceuticals GmbH

Inventor： Nicole Faust ,  Silke Wissing ,  Nikola Strempel

IPC: C07K14/705 ,  C07K14/71 ,  C07K19/00 ,  C12N5/07 ,  C12N5/071 ,  C12N5/10 ,  C12N5/16 ,  C12N15/09 ,  C12N15/113 ,  C12N15/62 ,  C12N15/63 ,  C12N15/85

CPC classification number: C12N15/62 ,  C07K14/70517 ,  C07K14/71 ,  C07K19/00 ,  C12N5/06 ,  C12N5/0625 ,  C12N5/10 ,  C12N5/16 ,  C12N15/09 ,  C12N15/1138 ,  C12N15/63 ,  C12N15/85 ,  C07K2319/00 ,  C12N2501/105

Abstract: The present invention relates to methods for the selection of a stable producer cell line expressing one or more protein(s) of interest (POIs), comprising the stable transfection of cells with a gene encoding a constitutively active variant of a growth factor receptor, respective methods for the expression of one or more POIs in such stable producer cell lines, and uses of a constitutively active variant of a growth factor receptor as selection marker in the cultivation of cells.

公开(公告)号：US20240247049A1

公开(公告)日：2024-07-25

申请号：US18443512

申请日：2024-02-16

Applicant: KEIO UNIVERSITY ,  NAGOYA INSTITUTE OF TECHNOLOGY

Inventor： Toshihide Kurihara ,  Yusaku Katada ,  Hiromitsu Kunimi ,  Kazuo Tsubota ,  Hideki Kandori

IPC: C07K14/72 ,  A61K31/7088 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P27/02 ,  C07K14/195 ,  C07K14/705 ,  C07K19/00 ,  C12N15/09 ,  C12N15/86

CPC classification number: C07K14/723 ,  A61K31/7088 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P27/02 ,  C07K14/195 ,  C12N15/09 ,  C12N15/86 ,  C07K14/705 ,  C07K19/00 ,  C12N2750/14143

Abstract: The purpose of the present invention is to provide an agent for restoring a visual function or an agent for preventing the deterioration in a visual function, which has an excellent visual function restoring ability. The agent for regenerating a visual function or the agent for preventing the deterioration in a visual function according to the present invention contains, as an active ingredient, a chimeric protein having both an amino acid sequence for a microorganism-origin ion-transporting receptor rhodopsin and an amino acid sequence for an animal-origin G-protein-coupled receptor rhodopsin. The chimeric protein is preferably one in which an amino acid sequence for a cytoplasm-side second loop and/or a cytoplasm-side third loop in the amino acid sequence for the microorganism-origin ion-transporting receptor rhodopsin is substituted by an amino acid sequence for a cytoplasm-side second loop and/or a cytoplasm-side third loop in the G-protein-coupled receptor rhodopsin.

公开(公告)号：US12031127B2

公开(公告)日：2024-07-09

申请号：US16091913

申请日：2017-04-05

Applicant: Cold Spring Harbor Laboratory

Inventor： Anthony Zador ,  Justus Kebschull

IPC: C12N15/10 ,  C07K14/18 ,  C12N5/0793 ,  C12N15/09 ,  C12Q1/6806 ,  C40B70/00

CPC classification number: C12N15/1065 ,  C07K14/1808 ,  C12N5/0619 ,  C12N15/09 ,  C12N15/10 ,  C12N15/102 ,  C12Q1/6806 ,  C40B70/00 ,  C12N2770/36143 ,  C12Q1/6806 ,  C12Q2522/101 ,  C12Q2525/161 ,  C12Q2535/122 ,  C12Q2563/179 ,  C12Q2565/514 ,  C12N15/102 ,  C12Q2535/122

Abstract: The present invention provides a composition comprising a plurality of labeled neurons, each of which is labeled by an expression construct that encodes a unique barcoded nucleic acid.

公开(公告)号：US12024714B2

公开(公告)日：2024-07-02

申请号：US16808170

申请日：2020-03-03

Applicant: NATIONAL CENTER FOR CHILD HEALTH AND DEVELOPMENT ,  SENJU PHARMACEUTICAL CO., LTD.

Inventor： Noriyuki Azuma ,  Taku Tanaka ,  Tadashi Yokoi

IPC: C12N5/079 ,  A61K35/30 ,  A61L27/00 ,  A61P27/06 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/6876 ,  G01N33/50

CPC classification number: C12N5/0621 ,  A61K35/30 ,  A61L27/00 ,  A61P27/06 ,  C12N15/09 ,  C12Q1/02 ,  C12Q1/6876 ,  G01N33/5058 ,  C12N2501/13 ,  C12N2506/02 ,  C12N2506/08 ,  C12N2506/45 ,  C12Q2600/136 ,  C12Q2600/158

Abstract: An object of the present invention is to provide a method of inducing pluripotent stem cells to differentiate into clinically applicable retinal ganglion cells.
The present invention provides a method for inducing pluripotent stem cells to differentiate into retinal ganglion cells that can be used for clinical application. Such method is a method for producing retinal ganglion cells with elongated axons comprising the following steps: (a) a step of inducing pluripotent stem cells to differentiate into retinal progenitor cells via floating culture; (b) a step of inducing the retinal progenitor cells obtained in step (a) to differentiate into retinal ganglion cells via floating culture; and (c) a step of allowing axons to elongate via adhesion culture of the retinal ganglion cells obtained in step (b).