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    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT
    1.
    发明申请
    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT 有权
    从PECAN NUT分离和分离WALNUT的方法

    公开(公告)号：US20100209566A1

    公开(公告)日：2010-08-19

    申请号：US12524735

    申请日：2008-01-30

    申请人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    发明人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    IPC分类号： G01N33/02

    CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

    摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

    摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及最近在日本属于菊科植物的可食用坚果的山核桃进口量的增加，开发了检测核桃的方法 迫切需要有歧视的手段。 对于叶绿体matK基因与表观基因序列的关注，我们开发了一种特异性检测菊科植物坚果的PCR方法，并确定了山核桃坚果的matK序列，建立了一种用核桃和山核桃相互鉴别核桃和山核桃的方法 用于区分核桃和山核桃果仁的酶。