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    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT
    1.
    发明申请
    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT 有权
    从PECAN NUT分离和分离WALNUT的方法

    公开(公告)号：US20100209566A1

    公开(公告)日：2010-08-19

    申请号：US12524735

    申请日：2008-01-30

    申请人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    发明人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    IPC分类号： G01N33/02

    CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

    摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

    摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及最近在日本属于菊科植物的可食用坚果的山核桃进口量的增加，开发了检测核桃的方法 迫切需要有歧视的手段。 对于叶绿体matK基因与表观基因序列的关注，我们开发了一种特异性检测菊科植物坚果的PCR方法，并确定了山核桃坚果的matK序列，建立了一种用核桃和山核桃相互鉴别核桃和山核桃的方法 用于区分核桃和山核桃果仁的酶。

    Primers and method of detecting bacteria
    3.
    发明授权
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号：US07326779B2

    公开(公告)日：2008-02-05

    申请号：US10491449

    申请日：2002-10-11

    申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

    摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

    Primers and method of detecting bacteria
    4.
    发明申请
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号：US20050176001A1

    公开(公告)日：2005-08-11

    申请号：US10491449

    申请日：2002-10-11

    申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    IPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

    摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

    Method for determining remaining lifetime
    5.
    发明授权
    Method for determining remaining lifetime 有权
    确定剩余寿命的方法

    公开(公告)号：US09523740B2

    公开(公告)日：2016-12-20

    申请号：US14008849

    申请日：2012-03-30

    申请人： Motoyoshi Okumura ,  Koichi Ichikawa ,  Yasushi Matsukawa ,  Katsunori Maegawa ,  Toshihiro Yamada

    发明人： Motoyoshi Okumura ,  Koichi Ichikawa ,  Yasushi Matsukawa ,  Katsunori Maegawa ,  Toshihiro Yamada

    IPC分类号： G01R31/36

    CPC分类号： G01R31/3606 ,  G01R31/3658 ,  G01R31/3679

    摘要： A method includes discharging an assembled battery (10) formed of a plurality of batteries until a state of charge (SOC) of the assembled battery becomes smaller or equal to a lower limit of a predetermined normal use range used in charge and discharge control of the assembled battery, measuring the discharge capacity of each of a plurality of battery blocks in the discharge of the assembled battery, the plurality of batteries being divided into the plurality of battery blocks, calculating the capacity difference in the discharge capacity among the plurality of battery blocks, and determining the remaining life of the battery block or the assembled battery based on the calculated capacity difference.

    摘要翻译： 一种方法包括对由多个电池形成的组电池（10）进行放电，直到组电池的充电状态（SOC）变得小于或等于用于充电和放电控制的预定正常使用范围的下限 组装电池，测量组电池放电中的多个电池块中的每一个的放电容量，将多个电池分成多个电池块，计算多个电池块之间的放电容量的容量差 ，并且基于所计算的容量差来确定电池块或组合电池的剩余寿命。

    Shift indicator system for vehicle
    10.
    发明授权
    Shift indicator system for vehicle 失效
    车辆换档指示器系统

    公开(公告)号：US4853673A

    公开(公告)日：1989-08-01

    申请号：US890289

    申请日：1986-07-29

    申请人： Yoshinobu Kido ,  Toshihiro Yamada

    发明人： Yoshinobu Kido ,  Toshihiro Yamada

    IPC分类号： B60K23/00 ,  B60R16/02 ,  B60R16/023 ,  B60W10/04 ,  B60W10/10 ,  F16H59/24 ,  F16H59/36 ,  F16H59/42 ,  F16H59/48 ,  F16H61/00 ,  F16H61/02 ,  F16H63/40 ,  F16H63/42

    CPC分类号： B60R16/0236 ,  F16H63/42 ,  F16H2059/366 ,  F16H59/24 ,  F16H59/36 ,  F16H59/48 ,  F16H61/0213

    摘要： A shift indicator system for a vehicle comprises an engine speed sensor, an engine speed comparator for comparing the engine speed detected by the engine speed sensor with a preset value, an indicator which provides an indication for directing upshifting when the engine speed is not lower than the preset value, a delay circuit which inhibits the indicator from providing the indication for directing upshifting until the engine speed continues to be not lower than the preset value for a predetermined time interval, an engine speed increasing rate detector for detecting the rate of increase of the engine speed, an engine load increasing rate detector for detecting the rate of increase of the engine load, and a delay time setting circuit which receives the outputs of the engine speed increasing rate detector and the engine load increasing rate detector, and makes the predetermined time interval shorter as the engine speed increasing rate increases and longer as the engine load increasing rate increases.

    摘要翻译： 用于车辆的换档指示器系统包括发动机速度传感器，用于将由发动机转速传感器检测到的发动机转速与预设值进行比较的发动机转速比较器，当发动机转速不低于时提供指示换档指示的指示器 所述预设值，延迟电路，其禁止所述指示器提供用于引导升档的指示，直到所述发动机转速在预定时间间隔内持续不低于所述预设值;发动机速度增加率检测器，用于检测所述预设值， 发动机转速，用于检测发动机负荷增加率的发动机负载增加率检测器，以及接收发动机增速率检测器和发动机负荷增加率检测器的输出的延迟时间设定电路， 发动机转速增加率随着发动机负荷的增加而增加，时间间隔越短 吃了增加。