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    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT
    1.
    发明申请
    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT 有权
    从PECAN NUT分离和分离WALNUT的方法

    公开(公告)号：US20100209566A1

    公开(公告)日：2010-08-19

    申请号：US12524735

    申请日：2008-01-30

    申请人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    发明人： Takeo Yano ,  Yumiko Sakai ,  Kouji Uchida ,  Yoshiki Nakao ,  Kimie Ishihata ,  Shigeru Nakano ,  Toshihiro Yamada

    IPC分类号： G01N33/02

    CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

    摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.[Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

    摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及最近在日本属于菊科植物的可食用坚果的山核桃进口量的增加，开发了检测核桃的方法 迫切需要有歧视的手段。 对于叶绿体matK基因与表观基因序列的关注，我们开发了一种特异性检测菊科植物坚果的PCR方法，并确定了山核桃坚果的matK序列，建立了一种用核桃和山核桃相互鉴别核桃和山核桃的方法 用于区分核桃和山核桃果仁的酶。

    Primers and method of detecting bacteria
    3.
    发明授权
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号：US07326779B2

    公开(公告)日：2008-02-05

    申请号：US10491449

    申请日：2002-10-11

    申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

    摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

    Primers and method of detecting bacteria
    4.
    发明申请
    Primers and method of detecting bacteria 有权
    引物和检测细菌的方法

    公开(公告)号：US20050176001A1

    公开(公告)日：2005-08-11

    申请号：US10491449

    申请日：2002-10-11

    申请人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    发明人： Shigeru Nakano ,  Toru Kobayashi ,  Kenichi Funabiki ,  Yasuhiro Nagao ,  Toshihiro Yamada

    IPC分类号： C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/689 ,  C07K14/245 ,  C07K14/255 ,  C07K14/28 ,  C07K14/31 ,  C07K14/32 ,  Y02A50/451

    摘要： The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

    摘要翻译： 本发明的第一个引物是在适当条件下用于PCR的引物用于可检测地扩增大肠杆菌，沙门氏菌和弧菌属细菌的16S rRNA编码DNA，但在相同条件下用于PCR时， 不能用于可检测地扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。 本发明的第二个引物是在适当条件下用于PCR的引物用于可检测地扩增金黄色葡萄球菌和蜡状芽孢杆菌的16S rRNA编码DNA，但是当在相同条件下用于PCR时，不起作用 扩增编码叶绿体16S rRNA的DNA或线粒体16S rRNA编码DNA。

    Alcohol dehydrogenase gene of acetic acid bacterium
    6.
    发明授权
    Alcohol dehydrogenase gene of acetic acid bacterium 有权
    乙酸细菌的酒精脱氢酶基因

    公开(公告)号：US07354751B2

    公开(公告)日：2008-04-08

    申请号：US10548486

    申请日：2004-02-17

    申请人： Shigeru Nakano

    发明人： Shigeru Nakano

    IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12J1/04 ,  C12P7/54

    摘要： A novel gene having a function of improving acetic acid fermentation in practical level was cloned from a practical acetic acid bacterium belonging to the genus Gluconacetobacter by a method for obtaining a gene having a growth-promoting function in acetic acid-containing medium from the chromosomal DNA library of the acetic acid bacterium. It was made possible to significantly shorten the growth induction period and significantly improve the acetic acid fermentation rate of transformants obtained by introducing the gene into acetic acid bacteria, when such transformants are cultured in the presence of ethanol.

    摘要翻译： 通过从染色体DNA获得含有乙酸的培养基中具有生长促进功能的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平的乙酸发酵功能的新型基因 醋酸菌库。 通过在乙醇存在下培养这些转化体，可以显着缩短生长诱导期，显着提高通过将基因导入乙酸菌而得到的转化体的乙酸发酵率。

    Process for hot dip-coating steel material with molten aluminum alloy by one-stage coating method using flux and bath of molten aluminum alloy metal
    8.
    发明授权
    Process for hot dip-coating steel material with molten aluminum alloy by one-stage coating method using flux and bath of molten aluminum alloy metal 失效
    使用熔融铝合金金属助熔剂和浴液，通过一步涂布法将熔融铝合金热浸涂钢材的工艺

    公开(公告)号：US5853806A

    公开(公告)日：1998-12-29

    申请号：US822762

    申请日：1997-03-24

    申请人： Seijun Higuchi ,  Shigeru Nakano ,  Toshiyuki Aishima

    发明人： Seijun Higuchi ,  Shigeru Nakano ,  Toshiyuki Aishima

    IPC分类号： C23C2/02 ,  C23C2/12 ,  C23C2/30 ,  B05D3/04

    CPC分类号： C23C2/02 ,  C23C2/30

    摘要： Disclosed are a process for hot-dip coating a steel material with a molten aluminum alloy according to a one-stage coating method using a flux which comprises removing an oxide layer on a steel material surface, conducting activating treatment, then coating a chloride flux solution and dipping the steel material in a molten aluminum alloy coating bath floated a fluoride-containing flux, thereby coating the steel material surface with aluminum alloy, or comprises removing an oxide layer on a steel material surface, adjusting surface roughness, conducting activating treatment and dipping the steel material in a molten Al--Zn--Si alloy or Al--Si alloy coating bath floated a flux added an iron component, thereby coating the steel material surface with Al--Zn--Si alloy or Al--Si alloy to adhere a large amount thereof and a aluminum alloy coating bath.

    摘要翻译： 本发明公开了一种使用熔融金属的一次涂覆方法，使用熔融铝合金热浸涂覆钢材的方法，该焊剂包括除去钢材表面上的氧化物层，进行活化处理，然后涂覆氯化物助熔剂溶液 将钢材浸入熔融铝合金涂层中漂浮含氟化物的焊剂，由此用铝合金涂覆钢材表面，或者除去钢材表面的氧化物层，调整表面粗糙度，进行活化处理和浸渍 在熔融的Al-Zn-Si合金或Al-Si合金涂层中的钢材漂浮加入铁组分的助熔剂，由此用Al-Zn-Si合金或Al-Si合金涂覆钢材表面以大量粘附 和铝合金涂层浴。

    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria
    9.
    发明授权
    Gene participating in acetic acid tolerance, acetic acid bacteria bred using the gene, and process for producing vinegar with the use of the acetic acid bacteria 有权
    基因参与乙酸耐量，使用该基因繁殖的乙酸菌，以及使用乙酸菌生产醋的方法

    公开(公告)号：US07446192B2

    公开(公告)日：2008-11-04

    申请号：US10507446

    申请日：2003-03-12

    申请人： Hidetsugu Goto ,  Shigeru Nakano

    发明人： Hidetsugu Goto ,  Shigeru Nakano

    IPC分类号： C07H21/04

    CPC分类号： C12J1/04 ,  C07K14/195 ,  C12N1/20

    摘要： The present invention is intended to provide novel genes participating in acetic acid tolerance of acetic acid bacteria, and a method of improving acetic acid tolerance of microorganisms, particularly that of acetic acid bacteria by using the genes, further a method of efficiently producing vinegar with acetic acid at higher concentration by using acetic acid bacteria whose acetic acid tolerance is improved. In the present invention, novel genes having a function for improving acetic acid tolerance on practical level were cloned from practical acetic acid bacteria belonging to the genus Gluconacetobacter by a method of obtaining genes from chromosomal DNA library that enable to grow on the medium at a high concentration of acetic acid. Further, in transformants in which the genes were introduced into acetic acid bacteria, acetic acid tolerance was remarkably increased, and when the transformants are subjected to aeration culture in the presence of ethanol, the growth lag-time can be shortened, and the growth rate can also be improved, moreover the final acetic acid concentration can be remarkably improved.

    摘要翻译： 本发明旨在提供参与乙酸菌的乙酸耐受性的新基因，以及通过使用该基因来提高微生物，特别是乙酸菌的乙酸耐受性的方法，还提出了一种有效生产乙酸醋的方法 通过使用醋酸耐受性提高的乙酸菌，浓度较高的酸。 在本发明中，通过从染色体DNA文库获得能够在培养基上高生长的基因的方法，从属于葡糖型醋杆菌属的实用乙酸菌克隆了具有实用水平提高乙酸耐受性的新型基因 乙酸浓度。 此外，在将基因导入乙酸菌的转化体中，乙酸耐受性显着增加，当转化体在乙醇存在下进行通气培养时，可以缩短生长滞后时间，生长速度 也可以改善，而且可以显着提高最终的乙酸浓度。

    Method Of Breeding Cells To Improve Tolerance To Short Chain Fatty Acids
    10.
    发明申请
    Method Of Breeding Cells To Improve Tolerance To Short Chain Fatty Acids 审中-公开
    育种细胞方法提高短链脂肪酸的耐受性

    公开(公告)号：US20080138904A1

    公开(公告)日：2008-06-12

    申请号：US11793145

    申请日：2005-12-12

    申请人： Shigeru Nakano ,  Masahiro Fukaya

    发明人： Shigeru Nakano ,  Masahiro Fukaya

    IPC分类号： C12N15/09 ,  C12N1/21

    CPC分类号： C12P7/40 ,  C12N1/00 ,  C12N15/52 ,  C12P7/52 ,  C12P7/54

    摘要： It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.The present invention provides a method of breeding cells to improve tolerance to a short chain fatty acid, wherein a gene encoding a protein having a function of transporting a short chain fatty acid from the inside of cells to the outside of cells, is transformed into and expressed in the cells; a high cell-density culture method for producing useful substances using the cell; and a method of preparing a fermentation broth comprising a short chain fatty acid, wherein the cells are cultured under the conditions such that the cells produce a short chain fatty acid.

    摘要翻译： 旨在提供赋予微生物细胞等的方法，对包含甲酸和乙酸的短链脂肪酸的耐受性，其对生长有害，而不抑制细胞生长，同时保持高生产率 的有用物质。 还提供了在短链脂肪酸存在下有效培养微生物的方法，或者是通过发酵生产有用的短链脂肪酸的方法。 本发明提供一种育种细胞以改善对短链脂肪酸耐受性的方法，其中将编码具有从细胞内部输送到外部的短链脂肪酸的功能的蛋白质的基因转化到细胞外， 在细胞中表达; 用于使用该细胞产生有用物质的高细胞密度培养方法; 以及制备包含短链脂肪酸的发酵液的方法，其中所述细胞在使细胞产生短链脂肪酸的条件下培养。