公开(公告)号：US20160032257A1

公开(公告)日：2016-02-04

申请号：US14648957

申请日：2013-12-06

Applicant: UNIVERSITÄT HAMBURG

Inventor： Andrea Rentmeister ,  Daniela Stummer

IPC: C12N9/10 ,  C12P19/32

CPC classification number: C12N9/1007 ,  C12P19/32 ,  C12P19/34 ,  C12Y201/01

Abstract: An agent and method for modifying the 5′ cap of RNA, for example for the purposes of isolation and analysis. According to one aspect the invention provides modified enzymes, namely modified trimethylguanosine synthases 2 from Giardia lamblia (GlaTGS2), the enzymatic activity of which is changed such that as compared to wild type enzymes the former can use AdoMet analogues better as cofactors.

Abstract translation: 用于修饰RNA的5'帽的试剂和方法，例如用于分离和分析的目的。 根据一个方面，本发明提供了修饰的酶，即来自贾第鞭毛虫（GlaTGS2）的改性的三甲基鸟苷合酶2，其酶活性被改变，使得与野生型酶相比，前者可以更好地使用AdoMet类似物作为辅因子。