公开(公告)号：US20030228312A1

公开(公告)日：2003-12-11

申请号：US10423927

申请日：2003-04-28

Applicant: Yeda Research and Development Co. Ltd

Inventor： David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

IPC: A61K039/395 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06 ,  C07K016/24

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151 ,  C07K16/4258 ,  C07K2319/00

Abstract: Antibodies to Tumor Necrosis Factor receptors (TNF-Rs) which inhibit the cytocidal effect of TNF but not its binding to the TNF-Rs, and ligands interacting with other receptors of the TNF/NGF family, are provided together with methods of producing them. The antibodies preferably bind to the fourth cysteine rich domain of the p75 TNF receptor or to the region between said fourth cysteine rich domain and the cell membrane.

Abstract translation: 与TNF-NGF家族的其他受体相互作用的配体，抑制肿瘤坏死因子的杀细胞活性而不是与TNF-R结合的肿瘤坏死因子受体（TNF-Rs）的抗体与生产它们的方法一起提供。 抗体优选结合p75 TNF受体的第四半胱氨酸富集结构域或所述第四富含半胱氨酸的结构域与细胞膜之间的区域。

公开(公告)号：US20020111462A1

公开(公告)日：2002-08-15

申请号：US09800908

申请日：2001-03-08

Applicant: Yeda Research and Development Co., Ltd.

Inventor： David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

IPC: C07K014/705 ,  C12P021/02 ,  C07H021/04 ,  C12N005/06

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151 ,  C07K2319/00 ,  Y02A50/412

Abstract: Antibodies to tumor necrosis factor receptors (TNF-Rs) are disclosed together with methods of producing them. The antibodies are preferably those which inhibit the cytotoxic effect of TNF but not its binding to the TNF-Rs. Most preferably, the antibodies bind to an extracellular domain of the C-terminal cysteine loop of the p75 TNF receptor, which loop consists of the amino acid sequence Cys-185 to Thr-201 of SEQ ID NO:3.

Abstract translation: 公开了肿瘤坏死因子受体（TNF-Rs）的抗体及其生产方法。 抗体优选是抑制TNF的细胞毒作用但不与TNF-R结合的抗体。 最优选地，抗体结合p75 TNF受体的C-末端半胱氨酸环的细胞外结构域，该环由SEQ ID NO：3的氨基酸序列Cys-185至Thr-201组成。

公开(公告)号：US20030124675A1

公开(公告)日：2003-07-03

申请号：US10319536

申请日：2002-12-16

Applicant: Yeda Research and Development Co. Ltd.

Inventor： David Wallach ,  Hartmut Engelmann ,  Dan Aderka ,  Daniela Novick ,  Menachem Rubinstein

IPC: C12P021/02 ,  C12N005/06 ,  C07K016/24 ,  C07H021/04

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151

Abstract: Tumor Necrosis Factor (TNF) Binding Protein is isolated and substantially purified. It has the ability to inhibit the cytotoxic effect of TNF and/or to maintain its prolonged beneficial effects. Isolated cDNA encoding TNF Binding Protein II is obtained and may be used to produce expression vectors, prokaryotic or eukaryotic expression hosts and methods of obtaining the protein by culturing of such hosts.

Abstract translation: 肿瘤坏死因子（TNF）结合蛋白被分离并基本纯化。 它具有抑制TNF的细胞毒性作用和/或维持其延长的有益效果的能力。 获得编码TNF结合蛋白II的分离的cDNA，并可用于产生表达载体，原核或真核表达宿主以及通过培养这些宿主获得蛋白质的方法。

公开(公告)号：US20010019833A1

公开(公告)日：2001-09-06

申请号：US09800909

申请日：2001-03-08

Applicant: Yeda Research and Development Co. Ltd.

Inventor： David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

IPC: A61K039/395 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151 ,  C07K16/4258 ,  C07K2319/00

Abstract: Antibodies to Tumor Necrosis Factor receptors (TNF-Rs) which inhibit the cytocidal effect of TNF but not its binding to the TNF-Rs, and ligands interacting with other receptors of the TNF/NGF family, are provided together with methods of producing them. The antibodies preferably bind to the fourth cysteine rich domain of the p75 TNF receptor or to the region between said fourth cysteine rich domain and the cell membrane.

Abstract translation: 与TNF-NGF家族的其他受体相互作用的配体，抑制肿瘤坏死因子的杀细胞活性而不是与TNF-R结合的肿瘤坏死因子受体（TNF-Rs）的抗体与生产它们的方法一起提供。 抗体优选结合p75 TNF受体的第四半胱氨酸富集结构域或所述第四富含半胱氨酸的结构域与细胞膜之间的区域。

公开(公告)号：US20040161858A1

公开(公告)日：2004-08-19

申请号：US10632929

申请日：2003-08-04

Applicant: Yeda Research and Development Co., Ltd.

Inventor： David Wallach ,  Jacek Bigda ,  Igor Beletsky ,  Igor Mett ,  Hartmut Engelmann

IPC: G01N033/558

CPC classification number: C07K16/2866 ,  A61K38/00 ,  C07K14/7151 ,  C07K2319/00 ,  Y02A50/412

Abstract: Antibodies to tumor necrosis factor receptors (TNF-Rs) are disclosed together with methods of producing them and methods of use of such antibodies in immunoassays and purification of TBP-II by affinity chromatography. A diagnostic assay for endogenous antibodies to TBP-II is also disclosed.

Abstract translation: 公开了肿瘤坏死因子受体（TNF-Rs）的抗体及其制备方法以及免疫测定中使用此类抗体的方法，并通过亲和层析纯化TBP-II。 还公开了对TBP-II的内源性抗体的诊断测定。