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98 条记录 (耗时 0.022 秒)

    Membrane-Translocating Peptides
    91.
    发明申请
    Membrane-Translocating Peptides 有权
    膜 - 位移肽

    公开(公告)号：US20080287311A1

    公开(公告)日：2008-11-20

    申请号：US11996496

    申请日：2006-07-24

    申请人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    发明人： David Coomber ,  Kevin Fitzgerald ,  Duncan McGregor ,  Chris Ullman ,  Tomas Leanderson

    IPC分类号： C40B30/04 ,  C07K14/00 ,  C07H21/00 ,  C12N5/06

    CPC分类号： C12N15/1075

    摘要： A method is provided for selecting membrane-translocating peptides (MTPs) from a peptide display library that are capable of crossing or penetrating a lipid membrane. A plurality of nucleic acid constructs that encode displayed peptides are expressed, resulting in the formation of a plurality of nucleic acid-peptide complexes, each complex comprising at least one displayed peptide associated with the corresponding nucleic acid construct encoding the displayed peptide; the complexes are exposed to a population of membrane-encapsulated compartments, allowing a translocating reaction to occur; complexes that remain unassociated with the membrane are removed; optionally complexes that are associated with the membrane are removed; and internalised nucleic acid-peptide complexes are recovered. The membrane-encapsulated compartments may be artificial vesicles such as liposomes, or populations of one or more cell types.

    摘要翻译： 提供了从能够穿透或穿透脂质膜的肽展示文库中选择膜转位肽（MTP）的方法。 表达编码显示的肽的多个核酸构建体，导致形成多个核酸 - 肽复合物，每个复合物包含与编码所显示肽的相应核酸构建体相关联的至少一个显示的肽; 复合物暴露于膜包封的隔室群，允许发生易位反应; 与膜保持不相关的复合物被去除; 去除与膜相关的任选复合物; 并回收内化的核酸 - 肽复合物。 膜包封的隔室可以是人造囊泡，例如脂质体或一种或多种细胞类型的群体。

    Transcription factor RNA interference reagents and methods of use thereof
    94.
    发明申请
    Transcription factor RNA interference reagents and methods of use thereof 审中-公开
    转录因子RNA干扰试剂及其使用方法

    公开(公告)号：US20050197312A1

    公开(公告)日：2005-09-08

    申请号：US11070767

    申请日：2005-03-02

    申请人： Kevin Fitzgerald ,  Donald Jackson ,  Qi Guo

    发明人： Kevin Fitzgerald ,  Donald Jackson ,  Qi Guo

    IPC分类号： A61K48/00 ,  C12N9/00 ,  C12N15/113 ,  C12N15/85

    CPC分类号： C12N15/1137 ,  C12N15/113 ,  C12N2310/14

    摘要： The present invention concerns methods and reagents useful in modulating transcription factor gene expression in a variety of applications, including methods of use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siRNA), short interfering RNA (siRNA), and doublestranded RNA (dsRNA) molecules capable of mediating RNA interference (RNAi) against E2F1, NFkB, CREB-1, and/or CDC2A gene expression, useful in the treatment of cell cycle disorders, inflammatory conditions, reproductive disorders, cancers and any other condition that responds to modulation of E2F1, NFkB, CREB-1, and/or CDC2A expression and/or activity.

    摘要翻译： 本发明涉及可用于调节多种应用中的转录因子基因表达的方法和试剂，包括在治疗，诊断，靶标验证和基因组发现应用中使用的方法。 具体而言，本发明涉及能够介导针对E2F1，NFkB，CREB-1的RNA干扰（RNAi）的短干扰核酸（siRNA），短干扰RNA（siRNA）和双链RNA（dsRNA）分子的小核酸分子， 1和/或CDC2A基因表达，其可用于治疗细胞周期障碍，炎性病症，生殖障碍，癌症以及对E2F1，NFkB，CREB-1和/或CDC2A表达的调节作出响应的任何其它病症和/或 活动。

    Emergency tool and system
    95.
    发明申请
    Emergency tool and system 审中-公开
    紧急工具和系统

    公开(公告)号：US20050087045A1

    公开(公告)日：2005-04-28

    申请号：US10695321

    申请日：2003-10-27

    申请人： Piotr Gryciuk ,  Kevin Fitzgerald

    发明人： Piotr Gryciuk ,  Kevin Fitzgerald

    IPC分类号： B25B13/06 ,  B25B15/00

    CPC分类号： B25B13/065 ,  B25B15/004

    摘要： A single tool T-shaped tool terminates at the bottom of its vertical portion with a first rectangular slot or socket for holding a nested and detachable socket for turning a rectangular valve lever arm engaged therein. Each of the first and second rectangular slot and socket mate with a corresponding gas and water supply valve levers wherein rotating the horizontal cross-arm of the T section opens or closes the corresponding supply line. The first and second rectangular components that define the valve lever arm engaging sockets are each marked with a different surface intended to correspond with surface marking on the supply line or valve lever to facilitate easy and rapid identification of the valve in emergencies.

    摘要翻译： 单个工具T形工具在其垂直部分的底部终止于第一矩形槽或插座，用于保持嵌套和可拆卸的插座，用于转动接合在其中的矩形阀杆臂。 第一和第二矩形槽和插座中的每一个与相应的气体和水供应阀杆配合，其中旋转T部分的水平横臂打开或关闭对应的供应管线。 限定阀杆臂接合插座的第一和第二矩形部件各自标有不同的表面，旨在对应于供应管线或阀杆上的表面标记，以便于在紧急情况下容易且快速地识别阀。