公开(公告)号：US20080102449A1

公开(公告)日：2008-05-01

申请号：US11321984

申请日：2005-12-29

申请人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

发明人： Jason Trama ,  Martin E. Adelson ,  Eli Mordechai

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6895 ,  C12Q1/689

摘要： Methods and compositions useful in the detection and identification of species of Candida are disclosed. These species include Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, each of which is a causative agent for vaginal candidiasis. The compositions of the invention are combinations of oligonucleotides. These oligonucleotides include pairs of forward and reverse primers for polymerase chain reactions, wherein each primer pair is capable of priming the synthesis of an amplicon specific to one of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, but preferably is not capable of priming the synthesis of an amplicon specific to any of the other three species. In preferred embodiments, the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. These unique primer sequences account for the species specificity of the resultant amplicons. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. In preferred methods of the invention, a biological sample is tested for the presence of at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis by isolating nucleic acid from the sample, attempting a polymerase chain reaction in a mixture containing this nucleic acid and a plurality of these primer pairs, ascertaining whether any amplicon is produced in the mixture using an oligonucleotide probe, and determining the sequence of any resultant amplicon using the sequencing primers. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.

公开(公告)号：US20060246423A1

公开(公告)日：2006-11-02

申请号：US11343858

申请日：2006-01-31

申请人： Martin Adelson ,  Eli Mordechai

发明人： Martin Adelson ,  Eli Mordechai

IPC分类号： C12Q1/70 ,  G01N33/569 ,  G01N33/554 ,  A61M35/00 ,  G06F19/00 ,  C12M1/34 ,  C12Q1/04

CPC分类号： C12Q1/04

摘要： A method and kit related thereto are described for the collection and maintenance of detectability of a plurality of species of microbiological agents selected from the group consisting of bacteria, fungi, viruses, and protozoa, in a single gynecological sample which comprises providing transport media in a resealable container and instructions for preparation and handling of the gynecological sample and a written indication of the detectability of a plurality of species.

摘要翻译： 描述了与其相关的方法和试剂盒，用于在单个妇科样品中收集和维持选自细菌，真菌，病毒和原生动物的多种微生物剂的可检测性，其包括在 可重复密封的容器以及妇科样品的制备和处理说明以及多种物种的可检测性的书面指示。